FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113768
Total Bases16.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6060.532662963223402No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA5600.4922298009985233No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC5380.4728922016735813No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA5340.4693762745235919No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG5220.4588284930736235No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5120.4500386751986499No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5070.4456437662611631No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA4530.3981787497363055No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG4220.3709303143238872No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG4080.3586245692989241No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG3900.3428028971239716No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA3810.3348920610364953No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA3760.33049715209900854No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA3430.3014907531115955No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2900.2549047183742353No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC2760.24259897334927222No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2680.23556711904929328No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2560.22501933759932494No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC2500.21974544687434075No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2450.21535053793685396No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG2380.2091976654243724No Hit
GTACATGGGCGTCTCAGGTCGGTCTCACAGC2300.2021658111243935No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA2200.19337599324941987No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA2180.19161802967442515No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA2100.18458617537444624No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1990.17491737571197524No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1930.16964348498699106No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT1850.16261163068701218No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1810.1590957035370227No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1800.15821672174952536No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA1800.15821672174952536No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC1700.1494269038745517No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1690.14854792208705433No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1640.14415301314956752No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1640.14415301314956752No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1630.14327403136207018No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT1520.13360523169959918No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1510.13272624991210183No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA1510.13272624991210183No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC1480.13008930454960974No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC1480.13008930454960974No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC1450.12745235918711764No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG1450.12745235918711764No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA1420.12481541382462556No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1400.12305745024963083No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1390.12217846846213347No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG1380.12129948667463611No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCG1370.12042050488713875No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1350.11866254131214403No Hit
CTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG1290.11338865058715983No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC1260.11075170522466773No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC1250.10987272343717037No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA1240.10899374164967301No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC1220.1072357780746783No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1220.1072357780746783No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1190.1045988327121862No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT1170.10284086913719148No Hit
CCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTT1170.10284086913719148No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA1160.10196188734969411No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA1160.10196188734969411No Hit
CGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG1150.10108290556219675No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1140.10020392377469939No Hit
CCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAG1140.10020392377469939No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1140.10020392377469939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)