FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380829
Total Bases42.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA24890.653574176336361No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC24430.6414952642787183No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC23810.6252149915053737No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC21440.5629823359040409No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA19670.5165047829865899No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA17020.4469197461327788No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA16960.44534423586439054No Hit
GGTATCAACGCAGAGTACATGGGGG14930.392039471783924No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC14190.3726081784738032No Hit
GTATCAACGCAGAGTACATGGGGGG13980.3670938925344446No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA13900.3649932121765937No Hit
GTATCAACGCAGAGTACATGGGGGGG13880.36446804208713096No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG12770.33532110212194977No Hit
TATCAACGCAGAGTACATGGGGGGG12210.3206163396169935No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA11320.2972462706359022No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC9600.2520816429421079No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA8850.2323877645872557No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA8560.22477279829004618No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT8440.22162177775326983No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC8270.21715783199283667No Hit
GTATCAACGCAGAGTACATGGGGGGGACCTCC8160.21426939650079171No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA8030.210855790919284No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA7960.20901769560616443No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA7900.20744218533777628No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG7790.20455374984573127No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA7210.18932381725131228No Hit
TATCAACGCAGAGTACATGGGGGGGACCTCC7060.18538504158034183No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG6640.1743564697016246No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC6540.171730619254311No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT6470.16989252394119145No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA6170.1620149725992506No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC6150.16148980250978787No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC5870.1541374212573097No Hit
GTATCAACGCAGAGTACATGGGGAG5830.15308708107838426No Hit
GTATCAACGCAGAGTACATGGGGTG5760.15124898576526472No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA5630.147835380183757No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT5490.14415918955751794No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC5450.1431088493785925No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA5430.14258367928912977No Hit
GTATCAACGCAGAGTACATGGGATG5420.1423210942443984No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA5360.14074558397601022No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA5340.14022041388654752No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA5280.13864490361815934No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC5200.13654422326030843No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5150.13523129803665163No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC5100.13391837281299482No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT4990.13102993732094984No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA4980.13076735227621844No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA4770.12525306633685984No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC4760.12499048129212847No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT4660.12236463084481486No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA4650.12210204580008349No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG4600.1207891205764267No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG4550.11947619535276988No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT4480.11763810003965033No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA4440.1165877598607249No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG4340.11396190941341128No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG4300.11291156923448582No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC4270.11212381410029174No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC4260.11186122905556037No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT4140.10871020851878402No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC4130.10844762347405265No Hit
GTATCAACGCAGAGTACATGGGAGG4130.10844762347405265No Hit
GTATCAACGCAGAGTACATGGGATGCC4110.10792245338458993No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG4090.10739728329512721No Hit
ACGCAGAGTACATGGGGGGGACCTCC4060.10660952816093314No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3970.10424626275835086No Hit
TGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCA3950.10372109266888814No Hit
GGTATCAACGCAGAGTACATGGGGGGG3940.10345850762415677No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT3940.10345850762415677No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC3930.10319592257942542No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT3850.10109524222157451No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG3850.10109524222157451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)