FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924301
Total Bases129.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT292383.16325525992074No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA224342.4271314214741735No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC183321.983336597060914No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA163941.7736646395492377No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG151521.6392928277693088No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA75580.8176989963226265No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG67820.7337436614263102No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC63710.6892776270933386No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT57270.6196033543185607No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG55360.5989390901881531No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG53940.5835761294210435No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAG53890.583035180098258No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT51400.556095903823538No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA48990.5300221464652749No Hit
TAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCC48830.5282911086323611No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC48450.5241798937791909No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA46870.5070858951791679No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG45360.49074922563104445No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG44940.48620525131964587No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG43410.46965220204240826No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG43060.4658655567829095No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG42340.45807588653479764No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT40930.44282111563224535No Hit
GTACATGGGAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCC40210.4350314453841335No Hit
GAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC38770.4194521048879099No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG37870.4097150170777701No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTT37850.4094986373486559No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG37810.4090658778904275No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT37740.4083085488385277No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGA32170.34804679428021823No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA30580.330844605815638No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC29450.3186191511206847No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC29080.3146161261320717No Hit
GTACATGGGAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGC28420.30747559507130257No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA27850.30130877279154733No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA27800.3007678234687618No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG27500.29752212753204854No Hit
CGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACG27340.2957910896991348No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG27220.2944928113244495No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC25440.27523501543328416No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA24010.25976386480161767No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG23840.2579246371041468No Hit
GTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAC22630.2448336634927367No Hit
GTACATGGGAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21660.23433924663069713No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA21490.23250001893322628No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA19920.21551421019776026No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC19540.21140299534459012No Hit
ATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCAT18700.202315046721793No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC18120.1960400345774807No Hit
GTACATGGGAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTT17990.1946335663382383No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC17770.19225338931798192No Hit
CCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTT17730.19182062985975348No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC16980.18370639001797034No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT16700.1806770738103713No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT16690.1805688839458142No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA16440.1778641373318865No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCG16230.1755921501761872No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC16050.17364473261415925No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC15950.17256283396858815No Hit
TCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGC15910.17213007451035972No Hit
GGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC15770.1706154164065602No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC15710.16996627721921753No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA15600.16877618870908934No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG15370.16628782182427584No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATT15310.1656386826369332No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA14620.15817358198249273No Hit
GTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTGTTGGTTTT14370.155468835368565No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG14030.15179037997362332No Hit
GTACATGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAA13650.14767916512045318No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTG13560.1467054563394392No Hit
CGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG13290.1437843299963973No Hit
CTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGG13200.14281062121538332No Hit
GTACATGGGAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGG13180.1425942414862691No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA13070.1414041529761409No Hit
GGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCC13020.14086320365335533No Hit
GTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTA12590.13621103947739968No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC12540.13567009015461412No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC12500.1352373306963857No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG12350.1336144827280291No Hit
CTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATT12330.13339810299891486No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT12260.1326407739470151No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAG12190.13188344489511533No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGG11790.12755585031283098No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAA11750.12712309085460258No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA11320.12247092667864688No Hit
CAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG11240.12160540776219003No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAG11010.11911704087737654No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA10910.11803514223180545No Hit
TGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG10840.11727781317990568No Hit
GTATCAACGCAGAGTACATGGGGGG10800.11684505372167725No Hit
TATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTGTTGGTTTTC10800.11684505372167725No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG10650.11522220575332061No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG10540.11403211724319243No Hit
GCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCA10530.11392392737863533No Hit
GGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA10290.1113273706292647No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTA10160.10992090239002231No Hit
GAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC10070.10894719360900831No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA9930.10743253550520881No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC9850.10656701658875194No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA9840.10645882672419482No Hit
GTATCAACGCAGAGTACATGGGAGGAATAATGGAATAGGACCGCGGTTCT9740.10537692807862373No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT9620.10407864970393844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)