FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520022
Total Bases67.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC21060.40498286610951073No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG19540.3757533335128129No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC17050.3278707439300645No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC16850.32402475279892007No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA16490.31710196876286006No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG15510.29825661222025224No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14610.2809496521301022No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA14300.2749883658768283No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG14150.27210387252846996No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC12830.24672033106291658No Hit
GGTATCAACGCAGAGTACATGGGGG12700.24422043682767267No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA12490.240182146139971No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA12090.2324901638776821No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG12080.2322978643211249No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA11860.22806727407686597No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC10810.2078758206383576No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC9490.18249227917280425No Hit
GTATCAACGCAGAGTACATGGGGGG9440.1815307813900181No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG9190.17672329247608756No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8940.171915803562157No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA8600.1653776186392114No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC8100.1557626408113503No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT7990.15364734568922084No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC7840.1507628523408625No Hit
TATCAACGCAGAGTACATGGGGCTTGCT7750.1490321563318475No Hit
GTATCAACGCAGAGTACATGGGGCTTGCT7270.13980177761710083No Hit
GTTGTGGGGTGGTAAAACAGTGTTGTGGGGTGGTAAAACAGTGTTGTGGG6970.13403279092038414No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCG6660.12807150466711023No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA6480.12461011264908023No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA6230.11980262373514966No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG6200.119225725065478No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC6140.11807192772613467No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC5870.11287983969908966No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA5810.11172604235974633No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC5720.10999534635073131No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA5680.10922614812450243No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC5620.10807235078515909No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC5610.10788005122860186No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA5610.10788005122860186No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG5510.10595705566302964No Hit
CGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG5450.10480325832368631No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA5430.10441865921057185No Hit
GTATCAACGCAGAGTACATGGGATG5280.10153416586221352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)