FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176104
Total Bases31 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG19071.0828828419570253No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC12290.6978830690955344No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC11330.6433698269204561No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC10120.5746604279289511No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA8080.45881978830690956No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC7230.4105528551310589No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA6970.3957888520419752No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG6160.34979330395675284No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG5810.3299186844137555No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA5670.32196883659655656No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA5310.3015263707809022No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG5070.2878980602371326No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC4970.28221959751056197No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA4920.27938036614727657No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC4700.26688774814882116No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA4520.256666515240994No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA4450.2526915913323945No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA4330.24587743606050969No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA3970.2254349702448553No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC3940.22373143142688412No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA3860.21918866124562758No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA3800.2157815836096852No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT3690.20953527461045743No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG3640.2066960432471721No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG3580.20328896561122972No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3480.19761050288465906No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT3480.19761050288465906No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA3450.1959069640666879No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA3440.1953391177940308No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC3420.19420342524871667No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA3410.1936355789760596No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG3350.1902285013401172No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT3290.1868214237041748No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA3230.1834143460682324No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT3200.1817108072502612No Hit
GACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGC3150.1788715758869759No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG3140.1783037296143188No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG3110.17660019079634762No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG2870.16297188025257803No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC2820.16013264888929268No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA2810.15956480261663564No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCA2780.15786126379866441No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC2770.15729341752600737No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG2750.15615772498069322No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2700.1533184936174079No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA2690.15275064734475083No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT2650.15047926225412256No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG2610.1482078771634943No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC2550.1448007995275519No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAG2540.14423295325489482No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT2490.1413937218916095No Hit
AGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA2490.1413937218916095No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT2490.1413937218916095No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC2490.1413937218916095No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT2450.13912233680098124No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC2420.13741879798301004No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA2300.13060464271112524No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA2300.13060464271112524No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG2280.12946895016581111No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG2270.12890110389315404No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA2200.12492617998455459No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC2200.12492617998455459No Hit
AGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACG2200.12492617998455459No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT2200.12492617998455459No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT2170.1232226411665834No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2130.12095125607595512No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG2080.1181120247126698No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT2080.1181120247126698No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA2070.11754417844001272No Hit
TAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAG2060.11697633216735566No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT2030.11527279334938446No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG1980.11243356198609913No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT1970.11186571571344206No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC1940.11016217689547086No Hit
CAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTAT1930.10959433062281379No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1930.10959433062281379No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1930.10959433062281379No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC1920.10902648435015673No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG1910.10845863807749966No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT1900.1078907918048426No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1880.10675509925952846No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1870.1061872529868714No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC1860.10561940671421433No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1810.102780175350929No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA1800.10221232907827194No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC1800.10221232907827194No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1790.10164448280561486No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1780.1010766365329578No Hit
GAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGA1780.1010766365329578No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1780.1010766365329578No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC1770.10050879026030073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)