FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49525
Total Bases7.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2800.5653710247349824No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2770.5593134780413933No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA1960.3957597173144876No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1960.3957597173144876No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1850.3735487127713276No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1830.36951034830893487No Hit
GGTATCAACGCAGAGTACATGGGGG1780.35941443715295307No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC1520.3069156991418476No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1480.29883897021706207No Hit
GTATCAACGCAGAGTACATGGGGGG1340.270570418980313No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1260.2544169611307421No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC1120.22614840989399293No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1110.22412922766279655No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1050.21201413427561835No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1030.20797576981322566No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA1000.20191822311963653No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA930.18778394750126198No Hit
TATCAACGCAGAGTACATGGGGGGG920.1857647652700656No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC890.17970721857647654No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC880.17768803634528016No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA870.1756688541140838No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA860.17364967188288744No Hit
GTATCAACGCAGAGTACATGGGGGGG830.16759212518929834No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT780.1574962140333165No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG730.1474003028773347No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA710.14336193841494194No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA700.1413427561837456No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA690.1393235739525492No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG670.1352852094901565No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA650.13124684502776374No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA650.13124684502776374No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA640.1292276627965674No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG630.12720848056537104No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA620.12518929833417466No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT610.1231701161029783No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT610.1231701161029783No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC600.12115093387178194No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC580.1171125694093892No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT560.11307420494699646No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT560.11307420494699646No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT560.11307420494699646No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA550.1110550227158001No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG550.1110550227158001No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT550.1110550227158001No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG540.10903584048460374No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT540.10903584048460374No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA530.10701665825340737No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG530.10701665825340737No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG520.104997476022211No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG520.104997476022211No Hit
GTATCAACGCAGAGTACATGGGGAG510.10297829379101464No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT500.10095911155981827No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT500.10095911155981827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)