FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008157225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008157225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97185678
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG5386570.5542555354709776TruSeq Adapter, Index 3 (97% over 36bp)
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG3748650.3857204144832945No Hit
CTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCT2271470.23372476755268404No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA2149220.2211457535955041No Hit
CCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTT2081640.21419205410081102No Hit
CCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCAGT1943620.19999037306710973No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTATG1854930.19086454281874743TruSeq Adapter, Index 3 (97% over 36bp)
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1844070.18974709421690716No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCAC1642770.16903416571318255No Hit
CCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCAGTT1612900.1659606675790233No Hit
CTCAGGATCCACATGCAGCTTGTCACAGTGCAGTTCACTCAGCTGGGCAAA1606210.165272294545293No Hit
CCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTC1584320.163019905052265No Hit
CGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG1537970.15825068380960414No Hit
CTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAA1508040.15517101192626345No Hit
CCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCTTG1450480.14924832854487058No Hit
CCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTG1441890.14836445345372803No Hit
GTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCA1397480.14379485010126697No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC1381970.14219893593786526No Hit
GCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGG1374670.14144779645412364No Hit
CCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAA1373130.14128933689180004No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAG1350010.1389103855405526No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGC1338610.13773737319607937No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACAC1328920.1367403127032771No Hit
GCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCA1306280.13441075134548117No Hit
CCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCT1257590.1294007538847442No Hit
GTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCAT1238060.12739119852618613No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCC1200290.1235048234164709No Hit
GTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGAC1193570.12281336350814984No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG1180190.12143661744068915No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTT1161630.11952687102723099No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAG1145760.11789391436874064No Hit
CACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACT1118210.11505913453626365No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTT1056120.1086703330916722No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAG1055760.10863329059658357No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA1045300.10755700032261954No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC1036190.10661961940523787No Hit
GGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAG1014260.10436311407942228No Hit
GTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTT1006050.10351833939976217No Hit
CACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAA973670.10018657275817945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)