FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008157623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008157623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102264
Total Bases14.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT39603.872330438864116No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA33913.3159274035828834No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG22332.18356410858171No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC21932.1444496597042946No Hit
GTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAC20902.0437299538449505No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15391.5049284205585542No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG11701.144097629664398No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA10891.0648908706876319No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG10190.9964405851521552No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG8720.852694985527654No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG8140.7959790346554018No Hit
GTACATGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACC8130.7950011734334662No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT7550.7382852225612141No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC7510.7343737776734726No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG7370.7206837205663772No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA6960.6805914104670265No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAG6280.6140968473754205No Hit
GAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC6050.5916060392709066No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5940.5808495658296174No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG5770.5642259250567159No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTT5700.5573808965031682No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5580.5456465618399436No Hit
TAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCC5490.5368458108425253No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT5410.5290229210670422No Hit
GTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAAT5120.5006649456309161No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC4960.48501916607994994No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG4780.46741766408511304No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGA4560.4459047172025346No Hit
TATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATA4240.41461315810060234No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC3660.3578972072283501No Hit
CGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACG3630.35496362356254396No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC3600.35203003989673787No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3490.34127356645544865No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATT3340.3266056481264179No Hit
GGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCC2850.2786904482515841No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2850.2786904482515841No Hit
GGTATCAACGCAGAGTACATGGGGG2790.2728232809199718No Hit
ATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCAT2740.2679339748102949No Hit
ACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGA2680.2620668074786826No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2650.2591332238128765No Hit
GAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG2600.25424391770319954No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2530.24739888914965188No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT2460.24055386059610417No Hit
GGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC2440.23859813815223344No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2350.229797387154815No Hit
GCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCA2250.22001877493546115No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA2040.19948368927481813No Hit
GAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC2030.19850582805288272No Hit
TCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT2010.19655010560901195No Hit
TCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGC1990.19459438316514122No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1940.18970507705546427No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG1900.18579363216772277No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1830.17894860361417506No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1770.17308143628256278No Hit
GTATCAACGCAGAGTACATGGGGAG1760.17210357506062737No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG1730.16916999139482125No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1710.16721426895095048No Hit
CAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG1670.16330282406320895No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA1670.16330282406320895No Hit
GTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTA1650.16134710161933818No Hit
TATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGCTAGGAATAA1600.15645779550966127No Hit
GTATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGCTAGGAATA1600.15645779550966127No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT1560.15254635062191974No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA1500.14667918329030744No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1500.14667918329030744No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT1490.14570132206837205No Hit
GGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGA1490.14570132206837205No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA1480.14472346084643667No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC1480.14472346084643667No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1460.14276773840256593No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC1430.13983415473675975No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA1420.1388562935148244No Hit
CTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGG1420.1388562935148244No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG1390.13592270984901822No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGG1380.13494484862708284No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG1370.13396698740514745No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGAC1350.13201126496127671No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA1350.13201126496127671No Hit
CCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG1340.1310334037393413No Hit
GTATCAACGCAGAGTACATGGGGGG1330.13005554251740592No Hit
ATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAATAA1330.13005554251740592No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATT1330.13005554251740592No Hit
ACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGC1310.12809982007353518No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1300.12712195885159977No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG1290.1261440976296644No Hit
ACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA1270.12418837518579365No Hit
TGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG1270.12418837518579365No Hit
GTACATGGGTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT1270.12418837518579365No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA1260.12321051396385824No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1260.12321051396385824No Hit
GAGTACATGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA1250.12223265274192287No Hit
GGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGGAA1240.12125479151998748No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1220.11929906907611672No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1190.11636548541031055No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1180.11538762418837518No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1170.1144097629664398No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1150.11245404052256903No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1150.11245404052256903No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG1120.1095204568567629No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1120.1095204568567629No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1120.1095204568567629No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1110.1085425956348275No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG1110.1085425956348275No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG1100.10756473441289212No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1090.10658687319095675No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1090.10658687319095675No Hit
GCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGA1090.10658687319095675No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1080.10560901196902137No Hit
GTACTGGTAGGCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAAC1070.10463115074708597No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA1070.10463115074708597No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1060.10365328952515059No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA1040.10169756708127982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)