FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008157663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008157663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234767
Total Bases37.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTATATGAATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATAC9830.4187130218471931No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG9370.39911912662341814No Hit
CTATATGAATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT9320.39698935540344255No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA9310.39656340115944744No Hit
CCATAAACCTGGAGTGACTATATGAATGCCCCCCACCCTACCACACATTC9120.3884702705235404No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA8500.3620611073958435No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG8430.35907942768787776No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC8400.35780156495589244No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT8160.3475786631000098No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT7840.3339481272921663No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC7770.3309664475842005No Hit
AACCTGGAGTGACTATATGAATGCCCCCCACCCTACCACACATTCGAAGA7350.31307636933640587No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC7280.3100946896284401No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT7280.3100946896284401No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA7160.3049832387004988No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA7140.3041313302125086No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6430.27388857888885576No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA6100.25983208883701714No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA6050.2577023176170416No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA6000.255572546397066No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA5950.25344277517709046No Hit
CCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGAC5920.25216491244510514No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG5760.2453496445411834No Hit
CCCTAGCATTACTTATATGACATGTCTCCATACCCATTACAATCTCCAGC5710.24321987332120784No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5550.23640460541728608No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGAATG5090.216810710193511No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA5050.21510689321753057No Hit
CCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAA4960.2112733050215746No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT4790.2040320828736577No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC4700.2001984946777017No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG4610.19636490648174573No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC4600.1959389522377506No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC4600.1959389522377506No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT4570.1946610895057653No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG4560.1942351352617702No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA4520.19253131828578973No Hit
CTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAGGA4490.1912534555538044No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC4440.18912368433382887No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA4330.18443818764988265No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT4320.18401223340588754No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA4310.18358627916189244No Hit
CCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACC4310.18358627916189244No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG4260.18145650794191687No Hit
TAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGAATGCCCCCCA4230.18017864520993154No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC3830.16314047545012714No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG3820.16271452120613203No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC3800.1618626127181418No Hit
CATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCG3800.1618626127181418No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC3780.1610107042301516No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA3710.1580290245221858No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA3660.15589925330221027No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA3630.15462139057022495No Hit
GCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCT3600.15334352783823962No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA3590.15291757359424452No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC3570.1520656651062543No Hit
GTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGAATGCCCCCCACC3540.15078780237426895No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG3540.15078780237426895No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA3500.14908398539828852No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT3430.14610230569032273No Hit
ACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC3410.14525039720233252No Hit
CCCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTTC3380.1439725344703472No Hit
CTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCG3370.14354658022635208No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC3350.14269467173836187No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA3310.14099085476238143No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC3310.14099085476238143No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA3290.14013894627439122No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA3260.13886108354240587No Hit
TCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATCTCCCCTTTT3220.13715726656642543No Hit
GGTATCAACGCAGAGTACATGGGGG3110.13247176988247922No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA3080.1311939071504939No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT3050.12991604441850857No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA3020.12863818168652325No Hit
CCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCA3010.12821222744252814No Hit
CATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAAGCTGGTTT3010.12821222744252814No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG2920.12437863924657214No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2890.1231007765145868No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC2870.12224886802659658No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2870.12224886802659658No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA2860.12182291378260146No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT2850.12139695953860637No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT2840.12097100529461124No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT2810.11969314256262593No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC2800.11926718831863081No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT2800.11926718831863081No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG2800.11926718831863081No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC2790.11884123407463569No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG2750.11713741709865526No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC2690.11458169163468461No Hit
GTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCTACGACCAACTCA2670.1137297831466944No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG2640.11245192041470906No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT2610.11117405768272372No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC2600.11074810343872862No Hit
GACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTC2580.10989619495073838No Hit
ACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGT2570.10947024070674329No Hit
ATATGAATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAA2570.10947024070674329No Hit
GATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA2560.10904428646274816No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA2520.10734046948676773No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT2510.10691451524277261No Hit
GTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGT2500.10648856099877752No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2500.10648856099877752No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT2480.1056366525107873No Hit
CTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGC2480.1056366525107873No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTATGTCTC2470.10521069826679218No Hit
GTATCAACGCAGAGTACATGGGGAG2470.10521069826679218No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGCA2460.10478474402279708No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA2460.10478474402279708No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG2440.10393283553480684No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC2430.10350688129081174No Hit
TGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTC2430.10350688129081174No Hit
GACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGC2420.10308092704681662No Hit
TTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCA2410.10265497280282153No Hit
ACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGT2380.10137711007083619No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2370.10095115582684107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)