FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008157685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008157685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98573
Total Bases13.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG13191.3380946100859261No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC11641.1808507400606656No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9911.0053462915808589No Hit
GTATCAACGCAGAGTACATGGGGGG7050.715205989469733No Hit
GTATCAACGCAGAGTACATGGGGAG6590.6685400667525591No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA4770.483905329045479No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4520.4585434145252757No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC4430.44941312529800254No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG4160.42202225761618295No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG3900.39564586651517153No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG3600.3652115690909275No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA3560.361153662767695No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA3400.3449220374747649No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT3400.3449220374747649No Hit
GTATCAACGCAGAGTACATGGAGAG3360.34086413115153236No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA3180.322603552696986No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT3130.3175311697929453No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT3120.3165166932121372No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT3100.31448774005052094No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT2990.3033284976616315No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG2930.2972416381767827No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT2840.2881113489495095No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2810.28506791920708513No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT2700.27390867681819564No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2620.2657928641717306No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT2430.24651780913637608No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA2410.24448885597475983No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA2380.24144542623233545No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC2330.23637304332829479No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG2320.23535856674748662No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG2320.23535856674748662No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG2260.22927170726263785No Hit
GTATCAACGCAGAGTACATGGGAGG2210.22419932435859719No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2200.22318484777778905No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT2180.22115589461617277No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA2160.2191269414545565No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2130.2160835117121321No Hit
GTTATAAGACTGACTAGCCCGATGGGCCTCCCAACGGGCCCTCCTCCCCT2010.20390979274243454No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG2000.20289531616162643No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT1980.20086636300001015No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC1950.19782293325758576No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA1920.19477950351516135No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1910.1937650269343532No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC1860.18869264403031258No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT1860.18869264403031258No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG1850.18767816744950444No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC1840.1866636908686963No Hit
GTACTGGTAGGCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAAC1830.18564921428788816No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1780.1805768313838475No Hit
GGTATCAACGCAGAGTACATGGGGGG1780.1805768313838475No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT1750.17753340164142312No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1730.17550444847980684No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT1720.17448997189899873No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG1710.1734754953181906No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG1690.17144654215657432No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT1660.16840311241414993No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT1660.16840311241414993No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG1620.16434520609091738No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1590.161301776348493No Hit
GGTATCAACGCAGAGTACATGGGGAG1590.161301776348493No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT1570.15927282318687674No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG1540.15622939344445233No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1520.15420044028283608No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG1520.15420044028283608No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC1480.15014253395960353No Hit
GTATCAACGCAGAGTACATGGGGAAG1470.14912805737879542No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC1460.14811358079798728No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG1450.14709910421717914No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC1430.14507015105556287No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC1430.14507015105556287No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT1430.14507015105556287No Hit
CCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTTCA1430.14507015105556287No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT1400.14202672131313848No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC1390.14101224473233037No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG1360.13796881498990596No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT1350.13695433840909782No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC1340.1359398618282897No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC1330.13492538524748157No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT1310.1328964320858653No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG1290.13086747892424902No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC1280.12985300234344088No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA1270.12883852576263277No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA1270.12883852576263277No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA1260.12782404918182463No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA1260.12782404918182463No Hit
CTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACC1230.12478061943940023No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT1210.12275166627778397No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA1190.12072271311616772No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG1170.11869375995455145No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG1170.11869375995455145No Hit
TATCAACGCAGAGTACATGGGGAAG1160.11767928337374331No Hit
TTATAAGACTGACTAGCCCGATGGGCCTCCCAACGGGCCCTCCTCCCCTC1150.11666480679293517No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC1140.11565033021212706No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG1120.11362137705051079No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA1110.11260690046970266No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT1110.11260690046970266No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT1110.11260690046970266No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA1110.11260690046970266No Hit
TATCAACGCAGAGTACATGGGAGAG1100.11159242388889452No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1080.10956347072727825No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC1080.10956347072727825No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC1080.10956347072727825No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT1070.10854899414647014No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1050.10652004098485386No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC1050.10652004098485386No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG1040.10550556440404574No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC1040.10550556440404574No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC1030.1044910878232376No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC1030.1044910878232376No Hit
GTATCAACGCAGAGTACATGGGGAAAG1020.10347661124242946No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA1010.10246213466162135No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT1010.10246213466162135No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC1010.10246213466162135No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG1010.10246213466162135No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC1000.10144765808081321No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG1000.10144765808081321No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT1000.10144765808081321No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA990.10043318150000508No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA990.10043318150000508No Hit
GTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGA990.10043318150000508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)