FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008157896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008157896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5334
Total Bases914 kbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC2043.824521934758155No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC500.9373828271466067No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA450.843644544431946No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC370.6936632920884889No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC310.5811773528308962No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC270.5061867266591676No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT260.4874390701162355No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT250.46869141357330335No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC200.3749531308586427No Hit
GTACATGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT200.3749531308586427No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC200.3749531308586427No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA190.35620547431571054No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT190.35620547431571054No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA190.35620547431571054No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG180.3374578177727784No Hit
GTACATGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCC170.31871016122984624No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT160.29996250468691416No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA160.29996250468691416No Hit
CTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCT160.29996250468691416No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC140.26246719160104987No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA140.26246719160104987No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC140.26246719160104987No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG120.22497187851518563No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA110.20622422197225346No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA110.20622422197225346No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT110.20622422197225346No Hit
TGTAAGCATAGTATAGCGGTAATGGTAATAGCGATCGCCCAGCCTAAGGA110.20622422197225346No Hit
GCCATGCACCACCACCCACGGAATCGAGAATTC110.20622422197225346No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG110.20622422197225346No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA110.20622422197225346No Hit
GTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCT110.20622422197225346No Hit
ACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAG100.18747656542932134No Hit
GGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA100.18747656542932134No Hit
GTTCTAAGCAGAATCCTAACCCTGCGAGGGTGGGGAGAAAATCAATGTTT90.1687289088863892No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT90.1687289088863892No Hit
ACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAATTC90.1687289088863892No Hit
GGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC90.1687289088863892No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC80.14998125234345708No Hit
GCTATGAGGTGATCCGTCCCCACACATGGGATGGGGGAGGCAGAGACTGG80.14998125234345708No Hit
ATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTA80.14998125234345708No Hit
GTACAACACTTCAACTCACCATATGCATAAACTGCAAAATTCTTCACCAA80.14998125234345708No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA80.14998125234345708No Hit
GCTCTGTGCTGGTACGCCTCATCCCTGCACCCAGGGGCACTGGCATCGTC80.14998125234345708No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA80.14998125234345708No Hit
CCATCATCCTGGCCAAGCTCTCCATCGTCCCCGTGCGCAGAGGCTACTGG80.14998125234345708No Hit
ATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG80.14998125234345708No Hit
GCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGC80.14998125234345708No Hit
GTATAGCGGTAATGGTAATAGCGATCGCCCAGCCTAAGGAAGGGAATGGA70.13123359580052493No Hit
GGAGAGAACACGGGGAGGTTTGTTTGTGTGAAAGGAAAGTAGTCCAGGCT70.13123359580052493No Hit
ATCCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCCC70.13123359580052493No Hit
GTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAATAATTGCA70.13123359580052493No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG70.13123359580052493No Hit
GCCCCTCAAAGCACCCTGCCTCAATGGAGACACCACCTGAGCTGCCCATC70.13123359580052493No Hit
TTACCTATCTTAAATCTTTCTGAGTTGGTAGTAGATTCATGTTCAAGGAA70.13123359580052493No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGC70.13123359580052493No Hit
GTTATATCCAAAACTGTTTTTCCCTCTCCCCTACCTTGTCCCCCCTATTA70.13123359580052493No Hit
GTTGAGACGGATTCTCTATGTTGCCCAGGCTGGTCTCAGGCTCCTGGGCT70.13123359580052493No Hit
ATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC60.11248593925759282No Hit
TCTATGTGGTGCTGGGTAGGCCAGGTTTCAGCATCGCAGACAAGAAGCGC60.11248593925759282No Hit
TCCTCCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAA60.11248593925759282No Hit
AATCTAGGGAGGTCTCCTCTCAGTCCCTCGGAGAGACCACCGCCCACCTC60.11248593925759282No Hit
CATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAG60.11248593925759282No Hit
TAATTGGGATTACAGCTCTCTTGTACAACACTTCAACTCACCATATGCAT60.11248593925759282No Hit
CCACAATATAAACCATAATTGGGATTACAGCTCTCTTGTACAACACTTCA60.11248593925759282No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG60.11248593925759282No Hit
CATCAAGGGAGTGCGCAAAAGCAATGCAGCAGAACGCAGGGGGCCTCTGT60.11248593925759282No Hit
TAGCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATATTT60.11248593925759282No Hit
ACCTCATCTCTACTAAAAATACAAAAATGAGCCAGGCGTGGTGGCACGCA60.11248593925759282No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG60.11248593925759282No Hit
ATTCTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACT60.11248593925759282No Hit
TCTGGGCTTTAGATCCACGCAAGGTCTCCTAAATACAAGTCACTGTCATG60.11248593925759282No Hit
ACTTAAGCAGTAAGCGTATTTAGGTAAAAGCAGTTGAATTATGTTAAATG60.11248593925759282No Hit
CTATTGTAGTTCCCAGATTCAATCCACCGAAGTGTTCACTGTCATCTGTT60.11248593925759282No Hit
ATCCACAGTGGCCACAGCTAACATCATTGCAGCACCTTTACTCCTTCGGC60.11248593925759282No Hit
ATATATGTGTGTGTTATAAATGTGGGTGCGTATGTTATATATGTGTGTGT60.11248593925759282No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT60.11248593925759282No Hit
CACCATATGCTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGTGCAAG60.11248593925759282No Hit
GTGTATACACAGGAATAAACTTTATGACATTT60.11248593925759282No Hit
GACTCCAGGCCCCTCCTTCCTGAATCATCAGCTGTGGGCATCAGGCCCAC60.11248593925759282No Hit
CTGCGGCTCTGTGCTGGTACGCCTCATCCCTGCACCCAGGGGCACTGGCA60.11248593925759282No Hit
CAGTAAAAATGACTCCCATCCATCCTCTGACAAGGAAGAAGGTGAGGCCA60.11248593925759282No Hit
TTGTAATGCTTGTACAGTTGATGTCGATGCTCACTGCTTCTGCTTTTTCT60.11248593925759282No Hit
CTGTAGTATTGGTGTCATGGGAATGTACGTTATTTCCAAAGTCATTTCAT60.11248593925759282No Hit
GGTGGACCCTTGGCCTGTAACAAGAATGGACTGTGGTATCAGATTGGAGT60.11248593925759282No Hit
CTATAAAATAAATCCTATTATTTTTCTCAGGAATCTGGTTAGGAATTGCA60.11248593925759282No Hit
GCGCAGGGCCAAGTTCAAGTTTCCTGGCCGCCAGAAGATCCACATCTCAA60.11248593925759282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)