FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26360
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2961.1229135053110775No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2590.9825493171471926No Hit
GTATCAACGCAGAGTACATGGGGGGG2340.8877086494688923No Hit
GTATCAACGCAGAGTACATGGGGGG1900.7207890743550834No Hit
TATCAACGCAGAGTACATGGGGGGG1890.7169954476479514No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1380.5235204855842186No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1370.5197268588770865No Hit
GGTATCAACGCAGAGTACATGGGGG1320.5007587253414264No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC990.3755690440060698No Hit
GTATCAACGCAGAGTACATGGGGAG900.34142640364188165No Hit
GGTATCAACGCAGAGTACATGGGGGGG790.29969650986342944No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT740.28072837632776937No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC680.25796661608497723No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA660.2503793626707132No Hit
GTATCAACGCAGAGTACATGGGGTG640.24279210925644917No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT640.24279210925644917No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG620.23520485584218512No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC580.22003034901365703No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT580.22003034901365703No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT570.21623672230652505No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT540.20485584218512898No Hit
GTATCAACGCAGAGTACATGGAGAG530.20106221547799696No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA520.19726858877086492No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC510.19347496206373294No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA510.19347496206373294No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA500.18968133535660092No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA490.18588770864946888No Hit
GGTATCAACGCAGAGTACATGGGGGG450.17071320182094082No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG440.1669195751138088No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC440.1669195751138088No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA410.15553869499241274No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA410.15553869499241274No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG400.15174506828528073No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA390.1479514415781487No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT390.1479514415781487No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG390.1479514415781487No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT380.1441578148710167No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG380.1441578148710167No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT380.1441578148710167No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC380.1441578148710167No Hit
GTATCAACGCAGAGTACATGGGAGG370.14036418816388468No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC360.13657056145675264No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC360.13657056145675264No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG360.13657056145675264No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC360.13657056145675264No Hit
GTATCAACGCAGAGTACATGGGATG350.13277693474962063No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG340.12898330804248861No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC340.12898330804248861No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG320.12139605462822459No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT320.12139605462822459No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT320.12139605462822459No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT320.12139605462822459No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA320.12139605462822459No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA310.11760242792109256No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA310.11760242792109256No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG310.11760242792109256No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC310.11760242792109256No Hit
GTATCAACGCAGAGTACATGGGGGGGACCTCC310.11760242792109256No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT300.11380880121396054No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT300.11380880121396054No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG300.11380880121396054No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT290.11001517450682852No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC290.11001517450682852No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG290.11001517450682852No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT280.10622154779969652No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT280.10622154779969652No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC280.10622154779969652No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA270.10242792109256449No Hit
GTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTG270.10242792109256449No Hit
TATCAACGCAGAGTACATGGGGAAG270.10242792109256449No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT270.10242792109256449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)