FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7615
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA871.1424819435325018No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC450.5909389363099147No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC340.4464871963230466No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC330.4333552199606041No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA290.3808273145108339No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC270.35456336178594877No Hit
CCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAAT270.35456336178594877No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC260.34143138542350626No Hit
GACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAG250.3282994090610637No Hit
CACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTA180.23637557452396585No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC180.23637557452396585No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT170.2232435981615233No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG160.21011162179908077No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC150.1969796454366382No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC150.1969796454366382No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA140.18384766907419567No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT130.17071569271175313No Hit
GTTAACACACTACCGAATGTGTCTTTAGATAGCCCTGTCCTGGTGGTATT130.17071569271175313No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA130.17071569271175313No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC130.17071569271175313No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT120.15758371634931057No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT120.15758371634931057No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG120.15758371634931057No Hit
GCCTTAGGACTCTGCAGAGAGTCCCCACCAGCAAAAAGACTCACCAGACG110.14445173998686803No Hit
GTTTAGAAGTGTAATTTAGGTATATTAGAGGAAGGAAGCTTCCCTTTATA110.14445173998686803No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT110.14445173998686803No Hit
GTTATGGTGCATTAAAATCCACATGGCAGCAAGTAGTAGCTGAGGCAGCA110.14445173998686803No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA110.14445173998686803No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA110.14445173998686803No Hit
GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC110.14445173998686803No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT100.13131976362442546No Hit
GCTCAGAAGATACCGTGAATCTAAGAAGATCGATCGCCACATGTATCACA100.13131976362442546No Hit
TCCTTAGAGAGAGTATAAAAATTGACTCTCAGCCAGGCACGGTGGCTCAC100.13131976362442546No Hit
GCATCAACCTTAAGATTTAAGGTTATTTGGGAGGCCAAGGTGGGCAGATC100.13131976362442546No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG100.13131976362442546No Hit
AACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGGAAAA100.13131976362442546No Hit
CACTCAAAAAGGACACTTCTGATTAAGACGGTTGAAACTAGAGATGGACA100.13131976362442546No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA100.13131976362442546No Hit
GATTAATAGGGCTTGCTTGGCAAGAGGAGACTGTATCCACTAGGGCACAG100.13131976362442546No Hit
GGGTGTAATAACAGTACCGGCCTTCTAGCATTGTTAGGGAGATTAAATGT100.13131976362442546No Hit
GGTAATCCCAGCACTTTGGGAGACCGAGGTGGGTGGATCACCTGAGGTCA100.13131976362442546No Hit
GTACTGTATTGGCTCTGTGAAAACATATTTGTGAAAAGAGTATGTAGTGG90.11818778726198292No Hit
GTAGTAGCCTAAAGTAATGAAAATATAGGGATCGCCTGGTGCGATGGCTC90.11818778726198292No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC90.11818778726198292No Hit
TGAAAACACAAATCATATGGAAGATGGGAACCCTCAGGCCTACCTCAACT90.11818778726198292No Hit
CCCCAGGGCACTGTGGCCAGGGTTCACATTGGCCAAGTTATCATGTCCAT90.11818778726198292No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG90.11818778726198292No Hit
GGAAAGTCCAAGCCGTAGGACCCAGTTTCCTTTCTTAGCTGATGTCTTTG90.11818778726198292No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA90.11818778726198292No Hit
CCTGTAATCCCAGAACTTTGGGAAGCCAAGACGGGTGGATCACCTGAGAT90.11818778726198292No Hit
GTCCAGGCCCAACCCCTCAATGTAGGCTTCTGTGAGCAGGAGTTTGAACA90.11818778726198292No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCA90.11818778726198292No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT90.11818778726198292No Hit
GTCCGGGGCATCCCAGTGCACGGCGTCCGTGGACCTGGGACCTCGAACCT80.10505581089954039No Hit
TCTGTAATCTCTTGGTCCTTAGGAAACTAACACTGGCTGGACATAGTGGC80.10505581089954039No Hit
ACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAA80.10505581089954039No Hit
GGCTGGGCACAATGGCTCACGCCTGTAATCCCAGCACTTTGAGAGGCCAA80.10505581089954039No Hit
CACTTTGGGAGGCTGAGGTGGGCAGATCACTTGAGGTCAGGAGTTCTGAG80.10505581089954039No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG80.10505581089954039No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC80.10505581089954039No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA80.10505581089954039No Hit
TCTCTACTAAAAATACAAAAATTAGCTAGGCATGGTGGCAGGCACCTGCA80.10505581089954039No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG80.10505581089954039No Hit
CACCTGTAATCCCAGAACTTTGGGAAGCCAAGACGGGTGGATCACCTGAG80.10505581089954039No Hit
CCTGTGGAGGCCTCCCGGGCCTGTCCTGGCCAATTTGCCTTTGCTTTGCA80.10505581089954039No Hit
ACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCC80.10505581089954039No Hit
CTTCCCCAGGAAGCCCTCGGCCCCCAAGAAGGGAGACAGTTCTGCTGAAG80.10505581089954039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)