FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38969
Total Bases6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG2890.7416151299751085No Hit
GTATCAACGCAGAGTACATGGGGGG2260.5799481639251712No Hit
GATACCTCTTTACGGACTCCACTTATGACTCCCTAAAGCCCATGTCGAAG2220.5696835946521593No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1720.4413764787395109No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1570.4028843439657164No Hit
CTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGC1230.31563550514511535No Hit
GTATCAACGCAGAGTACATGGGGAG1070.27457722805306783No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC960.24634966255228513No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC820.21042367009674356No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA740.1898945315507198No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA720.18476224691421386No Hit
CTAAACATTCTACTACTCACTCTCACTGCCCAAGAACTATCAAACTCCTG720.18476224691421386No Hit
GGTATCAACGCAGAGTACATGGGGGG670.171931535322949No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT660.16936539300469605No Hit
GTACTCTTAAAACTAGGCGGCTATGGTATAATACGCCTCACACTCATTCT640.1642331083681901No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC630.16166696604993713No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG600.15396853909517824No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTA590.15140239677692524No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC590.15140239677692524No Hit
GTATCAACGCAGAGTACATGGAGAG570.14627011214041932No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT540.13857168518566038No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA490.12574097359439554No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC480.12317483127614257No Hit
GTATCAACGCAGAGTACATGGGGAAG480.12317483127614257No Hit
GCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCC470.1206086889578896No Hit
GTATCAACGCAGAGTACATGGGAGG460.11804254663963663No Hit
AGATACCTCTTTACGGACTCCACTTATGACTCCCTAAAGCCCATGTCGAA460.11804254663963663No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG450.11547640432138366No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT430.11034411968487773No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA430.11034411968487773No Hit
AGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACTATGCA420.10777797736662474No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC410.10521183504837178No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT400.10264569273011881No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA390.10007955041186584No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC390.10007955041186584No Hit
GTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAAATGAATATTATCCCT390.10007955041186584No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT390.10007955041186584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)