FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88893
Total Bases14.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC8911.0023286423002935No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA8790.9888292666464176No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG7160.8054627473479351No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6460.7267163893669918No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5840.6569696151552991No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA5080.5714735693474177No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG4060.4567288762894716No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA3710.41735569729899996No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3650.41060600947206194No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA3530.3971066338181859No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC3410.3836072581643099No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA3170.35660850685655787No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA2760.3104856400391482No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG2730.3071107961256792No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC2670.3003611082987412No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA2510.28236194076023985No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG2480.2789870968467708No Hit
GTATCAACGCAGAGTACATGGGGGG2430.27336235699098915No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2390.26886256510636386No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT2340.26323782525058215No Hit
GGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAA2280.2564881374236441No Hit
GGTATCAACGCAGAGTACATGGGGG2200.24748855365439348No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2130.23961391785629915No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC2120.2384889698851428No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2060.23173928205820482No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT2000.2249895942312668No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1990.22386464626011046No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA1990.22386464626011046No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1980.22273969828895415No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1940.21823990640432878No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1870.21036527060623444No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC1850.2081153746639218No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG1770.19911579089467113No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG1680.18899125915426412No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1670.1878663111831078No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC1670.1878663111831078No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1670.1878663111831078No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1670.1878663111831078No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC1550.17436693552923177No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1540.17324198755807543No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA1540.17324198755807543No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1530.17211703958691912No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1510.16986714364460642No Hit
CCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG1490.16761724770229378No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC1470.1653673517599811No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1430.16086755987535578No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT1420.15974261190419942No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA1410.1586176639330431No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC1400.15749271596188677No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1400.15749271596188677No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA1390.15636776799073043No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT1380.1552428200195741No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1360.15299292407726142No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1360.15299292407726142No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1350.15186797610610508No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA1340.15074302813494878No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1340.15074302813494878No Hit
TAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAG1330.1496180801637924No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAA1330.1496180801637924No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG1330.1496180801637924No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1320.1484931321926361No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1310.14736818422147976No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1250.14061849639454177No Hit
GACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGC1250.14061849639454177No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1250.14061849639454177No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT1250.14061849639454177No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG1230.1383686004522291No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA1230.1383686004522291No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1230.1383686004522291No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT1220.13724365248107276No Hit
AGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA1180.1327438605964474No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT1170.13161891262529107No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1160.13049396465413476No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG1140.12824406871182206No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1120.12599417276950942No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC1120.12599417276950942No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT1080.12149438088488408No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1080.12149438088488408No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC1070.12036943291372774No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC1070.12036943291372774No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG1070.12036943291372774No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG1070.12036943291372774No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT1070.12036943291372774No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1050.11811953697141507No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG1040.11699458900025873No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1040.11699458900025873No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1030.11586964102910241No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA1020.11474469305794606No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG1010.11361974508678974No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1000.1124947971156334No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG980.11024490117332074No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA960.10799500523100806No Hit
TGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCA960.10799500523100806No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGCA960.10799500523100806No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT960.10799500523100806No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC950.10687005725985173No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA930.10462016131753907No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG920.10349521334638273No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT900.10124531740407007No Hit
AACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGA900.10124531740407007No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA890.10012036943291373No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC890.10012036943291373No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC890.10012036943291373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)