FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244271
Total Bases386.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG104280.46464976823208964No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT76730.34189275715811507No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC65130.2902055946006521No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC54850.2444000746790383No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG48920.2179772407164732No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA46410.20679320812860835No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC37680.16789416251424183No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA36250.1615223829920718No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT35740.1592499301554937No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC35090.1563536667363255No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC32760.1459716763260765No Hit
GCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCA29860.13304988568671075No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA28000.12476211651801408No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC27390.12204408469387164No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC27150.12097469512371724No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT26980.12021721084485787No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC26970.12017265294610142No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC26710.11901414757843416No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG26140.11647434734931743No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT25990.11580597886797094No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT25410.11322162074009778No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA25370.11304338914507205No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC25300.11273148385377701No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA24810.11054814681471176No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG24570.10947875724455737No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG24570.10947875724455737No Hit
CTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATA23930.1066270517241456No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC23400.10426548309005464No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG23290.10377534620373387No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC22850.10181479865845079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)