FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35310
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA6141.7388841687907108No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5231.4811668082696121No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5001.416029453412631No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3851.0903426791277258No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2700.7646559048428208No Hit
GGTATCAACGCAGAGTACATGGGGG2520.713678844519966No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2110.5975644293401303No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1700.4814500141602946No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA1590.45029736618521665No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1540.43613707165109034No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1500.4248088360237893No Hit
GTATCAACGCAGAGTACATGGGGAG1400.3964882469555367No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1330.3766638346077599No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA1290.36533559898045875No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1290.36533559898045875No Hit
GTATCAACGCAGAGTACATGGGGGG1190.3370150099122062No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1180.3341829510053809No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1150.32568677428490517No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1110.31435853865760405No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1080.3058623619371283No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1050.29736618521665253No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1030.291702067403002No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA1010.28603794958935147No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1010.28603794958935147No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA920.2605494194279241No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA920.2605494194279241No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG910.25771736052109884No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA890.25205324270744833No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA890.25205324270744833No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC870.2463891248937978No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT860.24355706598697255No Hit
GTATCAACGCAGAGTACATGGAGAG820.23222883035967146No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA820.23222883035967146No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC810.2293967714528462No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA790.2237326536391957No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC770.21806853582554517No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA770.21806853582554517No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG770.21806853582554517No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG760.21523647691871992No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC750.21240441801189466No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG750.21240441801189466No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA720.20390824129141888No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT680.19258000566411781No Hit
CTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCA680.19258000566411781No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT680.19258000566411781No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC670.18974794675729256No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC620.17558765222316625No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA620.17558765222316625No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT620.17558765222316625No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT620.17558765222316625No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG600.16992353440951571No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC590.16709147550269046No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT580.1642594165958652No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT580.1642594165958652No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG540.15293118096856415No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG520.1472670631549136No Hit
GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC520.1472670631549136No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC520.1472670631549136No Hit
GACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAG520.1472670631549136No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA510.14443500424808836No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC510.14443500424808836No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG510.14443500424808836No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA510.14443500424808836No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA500.1416029453412631No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT500.1416029453412631No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC490.13877088643443783No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC490.13877088643443783No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC490.13877088643443783No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT490.13877088643443783No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT490.13877088643443783No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT480.13593882752761258No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA480.13593882752761258No Hit
CACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTA480.13593882752761258No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC470.1331067686207873No Hit
ACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAAC460.13027470971396204No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG460.13027470971396204No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG460.13027470971396204No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT440.12461059190031153No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA440.12461059190031153No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC440.12461059190031153No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT440.12461059190031153No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG440.12461059190031153No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC440.12461059190031153No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG440.12461059190031153No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT430.12177853299348627No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC430.12177853299348627No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG430.12177853299348627No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT420.11894647408666101No Hit
CCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAG420.11894647408666101No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC420.11894647408666101No Hit
GTATCAACGCAGAGTACATGGGAGG420.11894647408666101No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA410.11611441517983573No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA410.11611441517983573No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC410.11611441517983573No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA410.11611441517983573No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT410.11611441517983573No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG400.11328235627301048No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT400.11328235627301048No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT390.11045029736618521No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT390.11045029736618521No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG380.10761823845935996No Hit
AAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAA370.10478617955253469No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC370.10478617955253469No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT370.10478617955253469No Hit
GGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATG370.10478617955253469No Hit
GGTATCAACGCAGAGTACATGGGGGG370.10478617955253469No Hit
TATCAACGCAGAGTACATGGGGAAAG360.10195412064570944No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT360.10195412064570944No Hit
GTATCAACGCAGAGTACATGGGGGGAAGATTTATAGGTAGAGGCGACAAA360.10195412064570944No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG360.10195412064570944No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG360.10195412064570944No Hit
TGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCA360.10195412064570944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)