FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296708
Total Bases216.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT69560.5364353424209614No Hit
GTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCTG61650.47543471621984285No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC57570.44397042356490435No Hit
GCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTC51400.3963883927607449No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA50270.38767401758915654No Hit
ATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTC45840.35351058218195613No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45200.34857500686353443No Hit
CCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGAC43030.3318403217995108No Hit
CCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTC42890.330760664698606No Hit
CTCATGCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGA39830.3071624452074021No Hit
GGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAA39700.30615990647084773No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA34640.26713801410957594No Hit
GGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCG31890.2459304639132326No Hit
CAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAG30410.23451694598938233No Hit
GCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAG29850.2301983175857633No Hit
CCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCC29120.22456867698818855No Hit
CCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTC29120.22456867698818855No Hit
CCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCG27920.2153144732661478No Hit
TCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATTATGCTAATGTT27610.2129238039712873No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24950.19241031905409697No Hit
CTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTAT24810.19133066195319223No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA23900.18431289079731134No Hit
CTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCC23770.18331035206075694No Hit
GAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCC23560.1816908664093998No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG23290.17960867057194063No Hit
GTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCT23200.17891460529278758No Hit
CTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTT22910.17667817272662775No Hit
GTCTTCTCATGCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCA22720.17521292380397127No Hit
CTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATG21830.16834938937679106No Hit
GCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTC21720.16750108736893735No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC21020.1621028018644136No Hit
GAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCC20840.16071467130610748No Hit
ACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGC19160.14775878609525045No Hit
CACGTACCCCGTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCA18070.1393528843810634No Hit
GCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGAC17720.13665374162880156No Hit
CCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTT17450.1345715457913424No Hit
GGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTA17110.13194952140343086No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16650.12840207664331524No Hit
CCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACG16460.12693682772065878No Hit
GAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGG16370.12624276244150573No Hit
GGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGGTCTGCACAACCACT15940.12292667277444114No Hit
CCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTG15630.1205360034795806No Hit
CTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCTGCCCTGGGCC15610.12038176675087991No Hit
GCTTGGCACGTACCCCGTGTACATACTTCCCGGGCGCCCAGCATGGAAAT15480.11937922801432552No Hit
TTCTCATGCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAA15430.1189936361925738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15330.1182224525490704No Hit
TGTTGGAGGAGAATGAATAAATAAAGTGAATCTTTGC15180.11706567708381532No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC15070.11621737507596158No Hit
CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTC15030.11590890161856023No Hit
TGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCTGC14900.11490636288200581No Hit
ACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGA14800.11413517923850242No Hit
TTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCA14760.11382670578110106No Hit
GGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC14540.11213010176539359No Hit
CCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATT14510.11189874667234259No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA14340.1105877344783868No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14340.1105877344783868No Hit
CCCCGGGCTCTCGCGGTCGCACGAGGATGCTTGGCACGTACCCCGTGTAC14270.11004790592793444No Hit
CCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACC14260.10997078756358408No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGG14040.10827418354787662No Hit
GGCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAATAAAGTGAATCT14020.10811994681917594No Hit
GCACGTACCCCGTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGC13820.10657757953216915No Hit
GTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCC13730.1058835142530161No Hit
CCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAG13710.10572927752431542No Hit
CCCCGTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGC13670.10542080406691406No Hit
GGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATG13660.10534368570256371No Hit
GCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCAC13400.10333860822945491No Hit
ACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTC13380.10318437150075423No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA13040.10056234711284268No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13010.10033099201979165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)