FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46930
Total Bases7.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5781.2316215640315364No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC5711.216705731941189No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5071.080332409972299No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA3220.686128276155977No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2800.596633283613893No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2370.5050074579160452No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1720.3665033027913914No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1290.27487747709354354No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1250.26635414447048794No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1230.26209247815896014No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1160.24717664606861284No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1160.24717664606861284No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1150.2450458129128489No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1100.2343916471340294No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA1090.2322608139782655No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1060.2258683145109738No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC1030.21947581504368208No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1020.2173449818879182No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1000.21308331557639038No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT930.19816748348604304No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT860.1832516513956957No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC830.176859151928404No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG830.176859151928404No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC800.17046665246111228No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC800.17046665246111228No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG740.15768165352652885No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT740.15768165352652885No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT730.15555082037076495No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC710.15128915405923715No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT710.15128915405923715No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC690.14702748774770935No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG680.14489665459194545No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG650.13850415512465375No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT650.13850415512465375No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC640.13637332196888985No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA630.13424248881312592No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA620.13211165565736202No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT610.12998082250159812No Hit
CCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAA590.12571915619007032No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT560.11932665672277862No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC560.11932665672277862No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT540.1150649904112508No Hit
GTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGG540.1150649904112508No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG530.1129341572554869No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC520.110803324099723No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA520.110803324099723No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG510.1086724909439591No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT500.10654165778819519No Hit
GGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTC500.10654165778819519No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA490.10441082463243127No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT490.10441082463243127No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT490.10441082463243127No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC470.10014915832090346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)