FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47438
Total Bases7.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6281.3238332138791686No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4430.9338504995994772No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3290.693536827016316No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2910.6134322694885956No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2050.4321430077153337No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1970.41527889034107673No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1840.38787469960790927No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1550.3267422741262279No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1530.3225262447826637No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1480.31198617142375307No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1360.2866899953623677No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1280.2698258779881108No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1270.2677178633163287No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1250.2635018339727645No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1240.2613938193009824No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1150.24242168725494329No Hit
GGTTCCAGCAGCTCAGGCTCCTTCCCATTGGTTCTCACAAAGTGTGCTTC1120.23609764323959695No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1060.22344955520890425No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1050.22134154053712213No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT1010.2129094818499937No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT990.20869345250642946No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA960.2023694084910831No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG940.19815337914751885No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC880.18550529111682618No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT840.17707323242969772No Hit
CTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGC820.17285720308613348No Hit
GGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGC810.17074918841435135No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG800.16864117374256923No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT800.16864117374256923No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT800.16864117374256923No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC770.1623171297272229No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT760.16020911505544078No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT760.16020911505544078No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT760.16020911505544078No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC740.15599308571187653No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC720.15177705636831232No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG720.15177705636831232No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC700.14756102702474808No Hit
ATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA660.13912896833761962No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT630.13280492432227328No Hit
GGTCTTCCGTATCTGATTGTTGCGTTTTTTAGTAAAACCAACACAGAACA620.1306969096504912No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA610.12858889497870907No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG610.12858889497870907No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG610.12858889497870907No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA600.12648088030692695No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC580.1222648509633627No Hit
GCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAACACCCTAGGC580.1222648509633627No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC570.1201568362915806No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT570.1201568362915806No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT560.11804882161979847No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC550.11594080694801637No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT550.11594080694801637No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA540.11383279227623425No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG540.11383279227623425No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA540.11383279227623425No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA540.11383279227623425No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC530.11172477760445212No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG530.11172477760445212No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG520.10961676293267No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG500.10540073358910579No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA500.10540073358910579No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG500.10540073358910579No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA490.10329271891732367No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC490.10329271891732367No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG490.10329271891732367No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT480.10118470424554155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)