FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67254
Total Bases11.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5880.8742974395574985No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5690.8460463318166949No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3060.4549915246676778No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2840.4222797157046421No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2570.38213340470455287No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG2300.3419870937044637No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2190.3256311892229458No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG2160.32117048800071374No Hit
TATCAACGCAGAGTACATGGGGAGTGGGCGGC2010.298866981889553No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT1970.29291938025991016No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1870.2780503761858031No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1790.26615517292651736No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT1760.2616944717042853No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1650.24533856722276742No Hit
GTATCAACGCAGAGTACATGGGGAGTGGGCGGC1600.2379040651857139No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1590.23641716477830316No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC1590.23641716477830316No Hit
TAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCA1580.23493026437089246No Hit
GTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTAT1510.22452196151901746No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1500.22303506111160673No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1490.22154816070419603No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1480.22006126029678533No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1470.21857435988937463No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG1430.21262675825973174No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1420.21113985785232106No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1360.2022184554078568No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1350.20073155500044607No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1330.19775775418562463No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC1300.1932970529633925No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT1280.1903232521485711No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1230.18288875011151753No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA1220.1814018497041068No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA1210.1799149492966961No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1200.1784280488892854No Hit
ATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACAT1200.1784280488892854No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG1170.17396734766705327No Hit
ACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC1160.17248044725964254No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1160.17248044725964254No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1130.1680197460374104No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1120.1665328456299997No Hit
AACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGA1120.1665328456299997No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA1110.165045945222589No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1110.165045945222589No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1100.16355904481517827No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1070.15909834359294614No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1050.1561245427781247No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC1050.1561245427781247No Hit
ACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGT1040.154637642370714No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG1040.154637642370714No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT1030.1531507419633033No Hit
ATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTC1030.1531507419633033No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT1020.15166384155589258No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCA1000.14869004074107117No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA990.14720314033366044No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC980.14571623992624974No Hit
CCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGAC960.1427424391114283No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT950.1412555387040176No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA950.1412555387040176No Hit
TGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTC930.13828173788919618No Hit
ACGCAGAGTACATGGGGAGTGGGCGGC930.13828173788919618No Hit
CTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATG910.13530793707437477No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT900.13382103666696404No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA890.13233413625955331No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC880.13084723585214264No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT880.13084723585214264No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC870.1293603354447319No Hit
GTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACC860.12787343503732118No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT860.12787343503732118No Hit
GACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATAC850.12638653462991048No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC840.12489963422249978No Hit
CCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCA830.12341273381508905No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG830.12341273381508905No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC830.12341273381508905No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT830.12341273381508905No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC820.12192583340767836No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC820.12192583340767836No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC820.12192583340767836No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG800.11895203259285694No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG800.11895203259285694No Hit
CTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCT790.11746513218544623No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA790.11746513218544623No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG770.11449133137062481No Hit
TTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCA770.11449133137062481No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC770.11449133137062481No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC770.11449133137062481No Hit
CCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTT760.11300443096321408No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC760.11300443096321408No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT750.11151753055580337No Hit
GCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCT740.11003063014839266No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA730.10854372974098195No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA730.10854372974098195No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT720.10705682933357123No Hit
TATCAACGCAGAGTACATGGGGGGCGGC720.10705682933357123No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC710.10556992892616053No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT710.10556992892616053No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC690.1025961281113391No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT690.1025961281113391No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC690.1025961281113391No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC690.1025961281113391No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT690.1025961281113391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)