FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73843
Total Bases8.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC3820.5173137602751784No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2510.33991035033787903No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2150.29115826821770513No Hit
TATCAACGCAGAGTACATGGGGGGG1940.2627195536476037No Hit
GTATCAACGCAGAGTACATGGGGGGG1870.25323998212423654No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1770.23969773709085493No Hit
GGTATCAACGCAGAGTACATGGGGG1610.2180301450374443No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1520.20584212450740083No Hit
GTATCAACGCAGAGTACATGGGGGG1480.20042522649404818No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1440.19500832848069555No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1420.1922998794740192No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1380.18688298146066656No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1340.18146608344731388No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1280.17334073642728492No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1270.17198651192394676No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA1260.1706322874206086No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1250.16927806291727043No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1100.14896469536719797No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG1040.140839348347169No Hit
GTATCAACGCAGAGTACATGGGGGGGACCTCC1020.13813089934049266No Hit
TATCAACGCAGAGTACATGGGGGGGACCTCC1010.1367766748371545No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA960.13000555232046368No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA950.12865132781712554No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT940.12729710331378735No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA930.1259428788104492No Hit
GTATCAACGCAGAGTACATGGGATG920.12458865430711102No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA900.1218802053004347No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA870.11781753179042022No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA860.11646330728708204No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG750.10156683775036225No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAA740.10021261324702409No Hit
GTATCAACGCAGAGTACATGGGAGG740.10021261324702409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)