FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10813
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC4654.300379173217424No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC1070.9895496162027189No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA1050.9710533616942568No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC610.5641357625080922No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC600.5548876352538611No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC590.54563950799963No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA590.54563950799963No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA580.536391380745399No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC520.48090261722001293No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA470.4346619809488579No Hit
GTACATGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT410.3791732174234717No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC410.3791732174234717No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC390.3606769629150097No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT350.32368445389808564No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA340.3144363266438546No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT330.3051881993896236No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT320.2959400721353926No Hit
GCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGC300.27744381762693054No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA290.2681956903726995No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG280.2589475631184685No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC250.23120318135577544No Hit
GTGTATGTACATATATATATTTTTTAATTTGATGAAAGCTGATTACTGTC230.2127069268473134No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG220.20345879959308238No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT220.20345879959308238No Hit
CGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGT210.1942106723388514No Hit
TCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTG210.1942106723388514No Hit
CATCACTCGAGACGTAAATTATGGCTGAATCATCCGCTACCTTCACGCCA210.1942106723388514No Hit
GAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC200.18496254508462037No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT190.17571441783038935No Hit
GTACATGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCC190.17571441783038935No Hit
CCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC190.17571441783038935No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGTGGACCACCTGAGTT180.16646629057615833No Hit
CCATGGAGAAATCCTGTTTCTACTAAAAATACAAAATTAGCCAGGTGTGG180.16646629057615833No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA180.16646629057615833No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC170.1572181633219273No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG160.1479700360676963No Hit
GGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAG160.1479700360676963No Hit
ATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG160.1479700360676963No Hit
CATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAG150.13872190881346527No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA150.13872190881346527No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG150.13872190881346527No Hit
AGACCAGCCTGACCACCATGGAGAAATCCTGTTTCTACTAAAAATACAAA140.12947378155923425No Hit
CTACTACACAGCGTTCTGAGCAGAGACACGCAGACCAGCTGAGGAGGACA140.12947378155923425No Hit
GCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCT140.12947378155923425No Hit
GCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACAC140.12947378155923425No Hit
CAGGTAGCCTGTGTGTTTGACAAACAGCAGCTGGCAGCGCTGCCTCCTGC140.12947378155923425No Hit
GTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCT140.12947378155923425No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC130.12022565430500323No Hit
CACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCG130.12022565430500323No Hit
TCATAGTATTCAAGCATCATTTATAGTTTGGTTTTGAGAACTTTTCTGGT130.12022565430500323No Hit
GTTGTATTCTTATTTCAGTGTTTCCTTCCAGACATTTTTAAGGTAATTGG130.12022565430500323No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC130.12022565430500323No Hit
GCGGAAGGGTACAGCCAATGCCCGAATGCCAGAGAAGGTCACATGGATGA130.12022565430500323No Hit
GGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT120.11097752705077223No Hit
GCTACTCAGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTC120.11097752705077223No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT120.11097752705077223No Hit
CCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACTTGAGGC120.11097752705077223No Hit
GGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA120.11097752705077223No Hit
ATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC120.11097752705077223No Hit
ATCCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCCC120.11097752705077223No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA120.11097752705077223No Hit
CGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCG120.11097752705077223No Hit
AATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCG110.10172939979654119No Hit
GTGTGGTAGTGCATGCCTGTAGTCCCAGCAACTCGGGAGGCTGAGGCAAG110.10172939979654119No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT110.10172939979654119No Hit
ACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAG110.10172939979654119No Hit
CTTCCTGCACATCTTGGCCTGTGCTCGCCTGAGCATCCGCCCGGGCCTGA110.10172939979654119No Hit
GTGTAACTGAGATAATTAATTTTGTCTGTAAAAATGGGAATAACTCACAG110.10172939979654119No Hit
GGATCATATCGTAAAAATAGAGATAAGTTGGGAAGATATATTGATTATGC110.10172939979654119No Hit
CTACTAAATATACAAAAATTAGACAGGCATGGTGGCGGCCGCCTGTAATC110.10172939979654119No Hit
GTGCTGACCCGCATCGGGCTGGAGACGTCACTGCGCTACGCCATCCAGCT110.10172939979654119No Hit
TTAACTGTGTATGTACATATATATATTTTTTAATTTGATGAAAGCTGATT110.10172939979654119No Hit
TTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTT110.10172939979654119No Hit
CATATATATGTGTGTGTTATAAATGTGGGTGCGTATGTTATATATGTGTG110.10172939979654119No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA110.10172939979654119No Hit
GTACATGGGGGAGGAAGACGCGGTCGTAAGGGCTGAGGATTTTTGGTCCG110.10172939979654119No Hit
GGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAC110.10172939979654119No Hit
CCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA110.10172939979654119No Hit
GTTATATATGTGTGTGTATACATAATATATACACACACCAGAGACAAAAA110.10172939979654119No Hit
ATATATGTGTGTGTATACATAATATATACACACACCAGAGACAAAAAATA110.10172939979654119No Hit
GGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCA110.10172939979654119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)