FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45248
Total Bases6.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5281.166902404526167No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4981.1006011315417257No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2970.6563826025459689No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2690.594501414427157No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2150.47515912305516267No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1870.41327793493635073No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1850.40885785007072134No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1660.3668670438472419No Hit
GGTATCAACGCAGAGTACATGGGGG1600.3536067892503536No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1550.34255657708628007No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1450.32045615275813294No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1430.3160360678925035No Hit
GTATCAACGCAGAGTACATGGGGGG1410.3116159830268741No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1380.30498585572842996No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1240.27404526166902404No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1200.2652050919377652No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1140.2519448373408769No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1100.24310466760961807No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC1070.236474540311174No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1040.22984441301272984No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT1030.22763437057991515No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC1010.2232142857142857No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT1010.2232142857142857No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC990.2187942008486563No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA950.20995403111739747No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC940.20774398868458277No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG930.20553394625176802No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC930.20553394625176802No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT910.20111386138613863No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG910.20111386138613863No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC880.19448373408769448No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG840.18564356435643564No Hit
GTATCAACGCAGAGTACATGGGGTG840.18564356435643564No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG820.18122347949080622No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG800.1768033946251768No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT780.17238330975954738No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA770.17017326732673269No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC770.17017326732673269No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT760.16796322489391796No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT740.16354314002828854No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG730.16133309759547382No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT730.16133309759547382No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA730.16133309759547382No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC720.15912305516265912No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT720.15912305516265912No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT710.1569130127298444No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC700.1547029702970297No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC700.1547029702970297No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG700.1547029702970297No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC650.14365275813295614No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT640.14144271570014144No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC630.13923267326732672No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT630.13923267326732672No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT630.13923267326732672No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA630.13923267326732672No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG620.13702263083451202No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC610.1348125884016973No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG610.1348125884016973No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT610.1348125884016973No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG600.1326025459688826No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA580.12818246110325318No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA580.12818246110325318No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG570.12597241867043846No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT560.12376237623762376No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA550.12155233380480904No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT550.12155233380480904No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT550.12155233380480904No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT540.11934229137199434No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA540.11934229137199434No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG540.11934229137199434No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT540.11934229137199434No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG530.11713224893917962No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT530.11713224893917962No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT530.11713224893917962No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC520.11492220650636492No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG510.11271216407355023No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT500.1105021216407355No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA500.1105021216407355No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC500.1105021216407355No Hit
GTATCAACGCAGAGTACATGGGGAG490.1082920792079208No Hit
CATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTT480.10608203677510608No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG480.10608203677510608No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT480.10608203677510608No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG480.10608203677510608No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG480.10608203677510608No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT470.10387199434229138No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA470.10387199434229138No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA470.10387199434229138No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC470.10387199434229138No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG470.10387199434229138No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT460.10166195190947666No Hit
GTTCAAGAGCTTGCGAACAAGACGGAAGAAGATATGATGAAAGTTCGAAA460.10166195190947666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)