FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90880
Total Bases10.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGGGG7370.8109595070422535No Hit
TATCAACGCAGAGTACATGGGGGGG6670.7339348591549295No Hit
GTATCAACGCAGAGTACATGGGGGG6660.7328345070422535No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5910.6503080985915493No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5760.6338028169014085No Hit
GGTATCAACGCAGAGTACATGGGGG5690.6261003521126761No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5210.5732834507042254No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA4670.5138644366197184No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3150.34661091549295775No Hit
GTATCAACGCAGAGTACATGGGGGGGACCTCC2540.2794894366197183No Hit
GTATCAACGCAGAGTACATGGGGTG2470.2717869718309859No Hit
TATCAACGCAGAGTACATGGGGGGGACCTCC2430.2673855633802817No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2410.26518485915492956No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA2200.24207746478873238No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2190.24097711267605632No Hit
GGTATCAACGCAGAGTACATGGGGGGG2140.23547535211267603No Hit
GTATCAACGCAGAGTACATGGGGAG2070.22777288732394366No Hit
GGTATCAACGCAGAGTACATGGGGGG2010.22117077464788734No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1850.2035651408450704No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1800.19806338028169013No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1790.1969630281690141No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1780.19586267605633803No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1700.18705985915492956No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1690.1859595070422535No Hit
ACGCAGAGTACATGGGGGGGACCTCC1600.17605633802816903No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1540.16945422535211266No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1480.16285211267605634No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1440.15845070422535212No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1390.15294894366197181No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG1330.1463468309859155No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1320.14524647887323944No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1270.13974471830985916No Hit
GTATCAACGCAGAGTACATGGGATG1240.13644366197183097No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1230.13534330985915494No Hit
GTATCAACGCAGAGTACATGGGAGG1220.13424295774647887No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG1210.1331426056338028No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1200.13204225352112675No Hit
TATCAACGCAGAGTACATGGGGATGGGG1200.13204225352112675No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1190.1309419014084507No Hit
GTATCAACGCAGAGTACATGGGATTG1180.12984154929577466No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1150.1265404929577465No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1130.12433978873239437No Hit
TATCAACGCAGAGTACATGGGATTG1130.12433978873239437No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1120.12323943661971831No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1090.11993838028169013No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1080.11883802816901408No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1050.11553697183098591No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG1030.1133362676056338No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1010.11113556338028169No Hit
GTATCAACGCAGAGTACATGGAGAG1010.11113556338028169No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1010.11113556338028169No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1000.11003521126760564No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC990.10893485915492958No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT980.10783450704225353No Hit
TATCAACGCAGAGTACATGGGGAAACTCC970.10673415492957747No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA940.10343309859154928No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC930.10233274647887323No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG930.10233274647887323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)