FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008158816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008158816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26530
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5652.1296645307199396No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4851.8281191104410102No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3401.2815680361854505No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2931.1044101017715793No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1980.7463249151903505No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1580.5955522050508858No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1490.5616283452695062No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1390.52393516773464No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1370.5163965322276668No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1290.4862419901997738No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1280.4824726724462872No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1260.47493403693931396No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1090.4108556351300414No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1010.3807010931021485No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT990.37316245759517525No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT960.36185450433471544No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA920.34677723332076893No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT900.3392385978137957No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT880.33169996230682247No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG770.29023746701846964No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG770.29023746701846964No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT750.28269883151149644No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA730.2751601960045232No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT700.2638522427440633No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC690.26008292499057667No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT680.25631360723709007No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC680.25631360723709007No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA660.24877497173011687No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC660.24877497173011687No Hit
GTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGG650.24500565397663024No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG650.24500565397663024No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG630.23746701846965698No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC600.22615906520919715No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC580.2186204297022239No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC570.21485111194873727No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT560.21108179419525064No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA560.21108179419525064No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG560.21108179419525064No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT550.20731247644176404No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG540.2035431586882774No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG540.2035431586882774No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC540.2035431586882774No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC520.1960045231813042No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG520.1960045231813042No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG510.19223520542781758No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT510.19223520542781758No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT500.18846588767433095No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC490.18469656992084432No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC490.18469656992084432No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC490.18469656992084432No Hit
GGGTCTCCGCTGTGGATCATCAGGCCATCCACAAACTTCATGGATTTAGC480.18092725216735772No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT480.18092725216735772No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC480.18092725216735772No Hit
ATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAG480.18092725216735772No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC470.1771579344138711No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA470.1771579344138711No Hit
TGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGG460.17338861666038446No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG450.16961929890689784No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG430.1620806633999246No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC430.1620806633999246No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG420.15831134564643798No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT420.15831134564643798No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT420.15831134564643798No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT420.15831134564643798No Hit
GTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACC420.15831134564643798No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC420.15831134564643798No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG410.15454202789295138No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG410.15454202789295138No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA400.15077271013946475No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC400.15077271013946475No Hit
GATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGC400.15077271013946475No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA390.14700339238597815No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT390.14700339238597815No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG390.14700339238597815No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT380.14323407463249152No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT380.14323407463249152No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG380.14323407463249152No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG380.14323407463249152No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG380.14323407463249152No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG370.13946475687900492No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC370.13946475687900492No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT360.1356954391255183No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT360.1356954391255183No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC360.1356954391255183No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG360.1356954391255183No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT360.1356954391255183No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT350.13192612137203166No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC350.13192612137203166No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT350.13192612137203166No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC340.12815680361854503No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT340.12815680361854503No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA340.12815680361854503No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA340.12815680361854503No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA340.12815680361854503No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC330.12438748586505843No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG330.12438748586505843No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA330.12438748586505843No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT330.12438748586505843No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA320.1206181681115718No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG320.1206181681115718No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA320.1206181681115718No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG320.1206181681115718No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC320.1206181681115718No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC310.11684885035808518No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA310.11684885035808518No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT310.11684885035808518No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC310.11684885035808518No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG310.11684885035808518No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG310.11684885035808518No Hit
CAGCACACCCTGTCTGAGCAACACGTGGCGCACAGCAGTGTCAACGTAGT300.11307953260459858No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG300.11307953260459858No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG300.11307953260459858No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG300.11307953260459858No Hit
TCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGGGGCGAGTATT300.11307953260459858No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC300.11307953260459858No Hit
GTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAA300.11307953260459858No Hit
CTCCTAAGGCAACAGAAACTACTGGGGCGAGTATTCCCAATGTATGGTCA300.11307953260459858No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT290.10931021485111195No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT290.10931021485111195No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT290.10931021485111195No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA290.10931021485111195No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA290.10931021485111195No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC290.10931021485111195No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT280.10554089709762532No Hit
GCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATA280.10554089709762532No Hit
TGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAAC280.10554089709762532No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT280.10554089709762532No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT280.10554089709762532No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA280.10554089709762532No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT270.1017715793441387No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG270.1017715793441387No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC270.1017715793441387No Hit
GGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATA270.1017715793441387No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA270.1017715793441387No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT270.1017715793441387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)