FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10763
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1121.0406020626219454No Hit
GGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGG1050.9755644337080739No Hit
GGCTTTTCACCCGCATTATCGCTACTCATGTCAGCATTCGCACTTCTGAT380.35306141410387437No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC340.3158970547245192No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC320.2973148750348416No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA260.24156833596580882No Hit
CTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTAC240.2229861562761312No Hit
GGACAACCTTCCGCGATCCGTCCCCCCATCGCATCATACGACGGTGCAGG220.2044039765864536No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC220.2044039765864536No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA210.19511288674161478No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA190.17653070705193719No Hit
GGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATCGCTAC190.17653070705193719No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA180.1672396172070984No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA180.1672396172070984No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC160.1486574375174208No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG160.1486574375174208No Hit
ATAACAATCTCCTCTGCTCTGCAGGCAGGAACAGTGTGGGTGAATTGCTA150.139366347672582No Hit
TTATCGCTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCATCCTT140.13007525782774318No Hit
GAGTTACCTACCAGGGCTATTCTCCCTACCAGCTCACCTGGGGCCGCCCA140.13007525782774318No Hit
GGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCT140.13007525782774318No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA140.13007525782774318No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCA140.13007525782774318No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG130.12078416798290441No Hit
GACATCCTTTGGTACCACCTAAAATAGTGTCTGAAGTAACATTTCAGTAT130.12078416798290441No Hit
ATACTATAAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGG130.12078416798290441No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC120.1114930781380656No Hit
GGATCCATCCTTATGATCCAAACACCTCCCACTAAACCCGACTTCCAACA120.1114930781380656No Hit
ATCCACCACGCTCGCGTGCTGATCCGCCAGCGCCATATCAGGGTCCGCAA110.1022019882932268No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA110.1022019882932268No Hit
GCATTCGCACTTCTGATACCTCCAGCATCCTTTACAAGACACCTTCGCAG110.1022019882932268No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA110.1022019882932268No Hit
GTCCCTTGAATTACGCACCCGGATTTGCCTAAGTGCCTTCTATGAACCAG110.1022019882932268No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC110.1022019882932268No Hit
GCAGAGATCGCAGTGAGCCAGGATCGAGCCACTGCACTCCAGCCTGGGCC110.1022019882932268No Hit
CGTCTTAAGAACAGGAAAAACGTTCCACGGGAATGGAGAGATTATCTATG110.1022019882932268No Hit
GAGATAATTACTGATAGGCAGTCTGGAAAGAAAAGAGGCTTTGGCTTTGT110.1022019882932268No Hit
CCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAG110.1022019882932268No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC110.1022019882932268No Hit
CTGTCAATATTAGATCAACGAGACAGAAAATGAACAAGGATATTCAGGAC110.1022019882932268No Hit
GGTTCCAAGAACATTAGAGGGAGAAGCCAGGGAGGAACATAGTTACACCC110.1022019882932268No Hit
GGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAA110.1022019882932268No Hit
GCTATTCCAACAGCTGGACAACCTTCCGCGATCCGTCCCCCCATCGCATC110.1022019882932268No Hit
AGCCTGAGCTAAGACACCAGAGTAATTAGGCAAGAAAAAGAAAGAAAAGG110.1022019882932268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)