FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42899
Total Bases7.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4030.9394158371990023No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3370.7855660971118207No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2840.662020093708478No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG2180.5081703536212966No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2010.4685423902655073No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC1960.45688710692556933No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1820.424252313573743No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC1670.389286463553929No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1670.389286463553929No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1550.3613137835380778No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1450.33800321685820184No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1270.29604419683442507No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1240.28905102683046224No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1220.28438891349448703No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1190.27739574349052426No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1120.26107834681461106No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1120.26107834681461106No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1110.25874729014662345No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1100.2564162334786359No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG1060.24709200680668547No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1060.24709200680668547No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1030.2400988368027227No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1000.23310566679875985No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG970.22611249679479706No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT960.2237814401268095No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC940.2191193267908343No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA890.2074640434508963No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA880.2051329867829087No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA870.2028019301149211No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA820.1911466467749831No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG790.1841534767710203No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA780.1818224201030327No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA770.1794913634350451No Hit
TAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAG740.1724981934310823No Hit
AGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACG740.1724981934310823No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC730.1701671367630947No Hit
GACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGC720.16783608009510712No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT690.16084291009114432No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC680.1585118534231567No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG680.1585118534231567No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG660.15384974008718152No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT660.15384974008718152No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT630.14685657008321873No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA630.14685657008321873No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC620.14452551341523112No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG600.13986340007925593No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT590.13753234341126833No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG590.13753234341126833No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC580.13520128674328072No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCA580.13520128674328072No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAG570.1328702300752931No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT570.1328702300752931No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA560.13053917340730553No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC550.12820811673931795No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG540.12587706007133034No Hit
GAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGA540.12587706007133034No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA530.12354600340334274No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC530.12354600340334274No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT520.12121494673535513No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA520.12121494673535513No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA510.11888389006736753No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT510.11888389006736753No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA510.11888389006736753No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC500.11655283339937993No Hit
AGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA490.11422177673139233No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG490.11422177673139233No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT480.11189072006340475No Hit
CTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGAT480.11189072006340475No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA470.10955966339541715No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC470.10955966339541715No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT460.10722860672742955No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC460.10722860672742955No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA450.10489755005944194No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC450.10489755005944194No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA440.10256649339145435No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA440.10256649339145435No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG440.10256649339145435No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT440.10256649339145435No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA430.10023543672346674No Hit
CCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAA430.10023543672346674No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT430.10023543672346674No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG430.10023543672346674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)