FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24540
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4221.719641401792991No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3471.4140179299103506No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2501.0187449062754685No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1940.7905460472697636No Hit
GGTATCAACGCAGAGTACATGGGGG1870.7620211898940505No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1780.7253463732681337No Hit
GTATCAACGCAGAGTACATGGGGGG1140.46454767726161367No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1140.46454767726161367No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1120.45639771801140994No Hit
GTATCAACGCAGAGTACATGGGGGGG1040.4237978810105949No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1000.40749796251018744No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT980.39934800325998365No Hit
GTATCAACGCAGAGTACATGGGGAG940.3830480847595762No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA940.3830480847595762No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC900.36674816625916873No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT880.35859820700896494No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG830.3382233088834556No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC810.3300733496332518No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG790.32192339038304807No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC770.31377343113284434No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA750.3056234718826406No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC750.3056234718826406No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA740.3015484922575387No Hit
TATCAACGCAGAGTACATGGGGGGG690.28117359413202936No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC690.28117359413202936No Hit
GTATCAACGCAGAGTACATGGGGTG660.26894865525672373No Hit
GTATCAACGCAGAGTACATGGAGAG630.2567237163814181No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG630.2567237163814181No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT580.23634881825590873No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC570.23227383863080683No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA570.23227383863080683No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG550.22412387938060307No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA540.2200488997555012No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT530.21597392013039934No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT510.20782396088019559No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT500.20374898125509372No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT500.20374898125509372No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT500.20374898125509372No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC480.19559902200488996No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT480.19559902200488996No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC480.19559902200488996No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT470.1915240423797881No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT450.18337408312958436No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG430.1752241238793806No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG420.1711491442542787No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG420.1711491442542787No Hit
GGTATCAACGCAGAGTACATGGGGAG420.1711491442542787No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT410.16707416462917685No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA400.16299918500407498No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC400.16299918500407498No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA400.16299918500407498No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT390.1589242053789731No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC380.15484922575387122No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG380.15484922575387122No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC380.15484922575387122No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC370.15077424612876936No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC360.1466992665036675No Hit
GGTATCAACGCAGAGTACATGGGGGG360.1466992665036675No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT350.14262428687856563No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG350.14262428687856563No Hit
GTATCAACGCAGAGTACATGGGATG340.13854930725346373No Hit
AGTTTACTGCGTCACGCTGAGGGACTCATCCTGGGCTACAATCCTATTGC330.13447432762836187No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA320.13039934800325997No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA320.13039934800325997No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT320.13039934800325997No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT320.13039934800325997No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG310.1263243683781581No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG310.1263243683781581No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT310.1263243683781581No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG310.1263243683781581No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG300.12224938875305623No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA300.12224938875305623No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC300.12224938875305623No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG300.12224938875305623No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG290.11817440912795436No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC290.11817440912795436No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC290.11817440912795436No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG290.11817440912795436No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA290.11817440912795436No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT290.11817440912795436No Hit
GGTATCAACGCAGAGTACATGGAGAG290.11817440912795436No Hit
ATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAG290.11817440912795436No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA290.11817440912795436No Hit
ATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTT280.11409942950285248No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA280.11409942950285248No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC280.11409942950285248No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT280.11409942950285248No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA270.1100244498777506No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT270.1100244498777506No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA270.1100244498777506No Hit
GTATCAACGCAGAGTACATGGGAGG270.1100244498777506No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC270.1100244498777506No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGA270.1100244498777506No Hit
ACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCT260.10594947025264873No Hit
GCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTT260.10594947025264873No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT260.10594947025264873No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC260.10594947025264873No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC260.10594947025264873No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC250.10187449062754686No Hit
ATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATTCTACCACGCC250.10187449062754686No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA250.10187449062754686No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT250.10187449062754686No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA250.10187449062754686No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG250.10187449062754686No Hit
TTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGA250.10187449062754686No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG250.10187449062754686No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC250.10187449062754686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)