FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25940
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1550.5975327679259831No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1320.5088666152659985No Hit
GGTATCAACGCAGAGTACATGGGGG970.37393986121819583No Hit
GTATCAACGCAGAGTACATGGGGGG860.3315343099460293No Hit
TATCAACGCAGAGTACATGGGATGGAAATC650.25057825751734775No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC630.24286815728604472No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA610.23515805705474174No Hit
CAATGACACCTACTCTGGGAAGCGTGAACCATTTTCTGGGGACCACAGTG560.21588280647648422No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA530.2043176561295297No Hit
GTATCAACGCAGAGTACATGGGATGGAAATC520.20046260601387816No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG490.18889745566692367No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA480.18504240555127216No Hit
GTATCAACGCAGAGTACATGGGGTG420.16191210485736315No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA390.15034695451040864No Hit
GAGACGCCGCCGCTGCGAAGGACCAATGAGAGCCCCGCTGCTACCGCCGG370.14263685427910563No Hit
GTATCAACGCAGAGTACATGGGATG360.13878180416345412No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT340.13107170393215112No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG330.12721665381649963No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC320.1233616037008481No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC320.1233616037008481No Hit
ACGCAGAGTACATGGGATGGAAATC320.1233616037008481No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT320.1233616037008481No Hit
GGTATCAACGCAGAGTACATGGGGGG320.1233616037008481No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC310.1195065535851966No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG290.1117964533538936No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT280.10794140323824211No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG280.10794140323824211No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG280.10794140323824211No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC280.10794140323824211No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA260.10023130300693908No Hit
GCCGGCGCCGGTGGTGCTGTCGCTCTTGATACTCGGCTCAGGCCATTATG260.10023130300693908No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT260.10023130300693908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)