FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34202
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4001.1695222501608094No Hit
GGTATCAACGCAGAGTACATGGGGG2840.8303607976141747No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2730.7981989357347523No Hit
GTATCAACGCAGAGTACATGGGGGG2610.7631132682299281No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2100.6139991813344249No Hit
TATCAACGCAGAGTACATGGGGGGG2090.6110753757090229No Hit
GTATCAACGCAGAGTACATGGGGGGG1740.508742178819952No Hit
GTATCAACGCAGAGTACATGGGGAG1290.377170925676861No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1080.3157710075434185No Hit
GTATCAACGCAGAGTACATGGGGTG1070.3128472019180165No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC930.27191392316238816No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT860.251447283784574No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA780.22805683878135782No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA760.22220922753055378No Hit
GTATCAACGCAGAGTACATGGGAGG750.21928542190515177No Hit
GGTATCAACGCAGAGTACATGGGGGG680.1988187825273376No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA670.19589497690193558No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT640.1871235600257295No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC620.18127594877492542No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT600.1754283375241214No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG570.16665692064791535No Hit
GGTATCAACGCAGAGTACATGGGGGGG560.1637331150225133No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC560.1637331150225133No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT540.15788550377170926No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG540.15788550377170926No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC520.15203789252090522No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA510.14911408689550318No Hit
GTATCAACGCAGAGTACATGGGATG500.14619028127010117No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA500.14619028127010117No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC480.14034267001929712No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT480.14034267001929712No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA470.13741886439389508No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA470.13741886439389508No Hit
GTATCAACGCAGAGTACATGGAGAG450.13157125314309104No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG450.13157125314309104No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA450.13157125314309104No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG440.12864744751768903No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG440.12864744751768903No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG440.12864744751768903No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG420.12279983626688497No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG420.12279983626688497No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG420.12279983626688497No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT390.11402841939067891No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG390.11402841939067891No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC380.11110461376527689No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC360.10525700251447284No Hit
GGTATCAACGCAGAGTACATGGGGTG350.1023331968890708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)