FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012487
Total Bases176.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT16070.15871808724457695No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA13780.1361005128954742No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT12630.12474234237081563No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG11950.1180262067562349No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT11760.11614963945216086No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG10910.10775446993393496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)