FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740552
Total Bases129.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT12320.16636238913675205No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA10370.14003067981721742No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT10180.13746502608864739No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG8970.12112586286985923No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT8320.11234862643001438No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG8260.1115384199894133No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT7600.10262614914280158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)