FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345743
Total Bases54.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA8100.23427806202873233No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC7850.22704725764512945No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG7510.21721336368342958No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC5290.15300382075703628No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA4790.1385422119898306No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4580.1324683363076042No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4500.1301544789048513No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA4170.12060981711849554No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4150.12003135276780731No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG4100.11858519189108672No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC3550.10267742224716045No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG3520.10180972572112813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)