FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21277
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4001.8799642806786672No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3711.7436668703294638No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3081.4475724961225738No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2491.1702777647224702No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2281.0715796399868402No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1710.8036847299901302No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1380.6485876768341402No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1310.6156883019222635No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1260.5921887484137802No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT990.4652911594679701No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG930.4370916952577901No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT910.4276918738543968No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC850.3994924096442168No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA810.3806927668374301No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA800.3759928561357334No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT780.3665930347323401No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT770.36189312403064344No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG760.35719321332894677No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC750.3524933026272501No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC740.3477933919255534No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA740.3477933919255534No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT700.3289937491187668No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT680.3195939277153734No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG680.3195939277153734No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC670.31489401701367675No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT630.2960943742068901No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG620.29139446350519344No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT610.28669455280349676No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC600.2819946421018001No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC590.2772947314001034No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG580.27259482069840674No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG570.26789490999671006No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA560.2631949992950134No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC540.2537951778916201No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT520.24439535648822672No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA520.24439535648822672No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT510.23969544578653004No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT500.2349955350848334No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC480.22559571368144005No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC480.22559571368144005No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT480.22559571368144005No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG450.21149598157635005No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC450.21149598157635005No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT440.20679607087465338No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC440.20679607087465338No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG430.20209616017295673No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC410.19269633876956338No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC410.19269633876956338No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA390.18329651736617006No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT380.17859660666447338No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC380.17859660666447338No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT380.17859660666447338No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT380.17859660666447338No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG380.17859660666447338No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA380.17859660666447338No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC370.1738966959627767No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC370.1738966959627767No Hit
CATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCT360.16919678526108006No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC360.16919678526108006No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG360.16919678526108006No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA350.1644968745593834No Hit
CTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG350.1644968745593834No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT350.1644968745593834No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG350.1644968745593834No Hit
TAATATGACAGGCCGCTAAAGACGTTCTACTCCGCCATATGAGATAATAT330.15509705315599004No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC330.15509705315599004No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC320.1503971424542934No Hit
AAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCA320.1503971424542934No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGA320.1503971424542934No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA320.1503971424542934No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC310.14569723175259672No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA310.14569723175259672No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG310.14569723175259672No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG310.14569723175259672No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA310.14569723175259672No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT300.14099732105090004No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG300.14099732105090004No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC300.14099732105090004No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG300.14099732105090004No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG290.13629741034920337No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC290.13629741034920337No Hit
TGTATGGTCAGAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTT290.13629741034920337No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA290.13629741034920337No Hit
GTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGG290.13629741034920337No Hit
TCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAG280.1315974996475067No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT280.1315974996475067No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA270.12689758894581005No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA270.12689758894581005No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG270.12689758894581005No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT260.12219767824411336No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA260.12219767824411336No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC260.12219767824411336No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT260.12219767824411336No Hit
CTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGC260.12219767824411336No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC260.12219767824411336No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA250.1174977675424167No Hit
TCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCG250.1174977675424167No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC250.1174977675424167No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC250.1174977675424167No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT240.11279785684072002No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA240.11279785684072002No Hit
GTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGCAA240.11279785684072002No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA240.11279785684072002No Hit
GAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTA240.11279785684072002No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG240.11279785684072002No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG240.11279785684072002No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT240.11279785684072002No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT240.11279785684072002No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG240.11279785684072002No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG240.11279785684072002No Hit
CTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATA240.11279785684072002No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT230.10809794613902336No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA230.10809794613902336No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG230.10809794613902336No Hit
GTGGTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTGGTAG230.10809794613902336No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG230.10809794613902336No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC230.10809794613902336No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG230.10809794613902336No Hit
GATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGT230.10809794613902336No Hit
ATAATATCCTTATCTTGAGACGCATAGCAAATGTAGGAGAGAGAGGTTAA230.10809794613902336No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG230.10809794613902336No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT220.10339803543732669No Hit
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC220.10339803543732669No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA220.10339803543732669No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT220.10339803543732669No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT220.10339803543732669No Hit
TCTCTGAACCTCAAAAATTATATGATTCATTAAAATTAAAGCCAGATGTT220.10339803543732669No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG220.10339803543732669No Hit
GTAAAGTGCTTCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGG220.10339803543732669No Hit
CCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCT220.10339803543732669No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT220.10339803543732669No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC220.10339803543732669No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC220.10339803543732669No Hit
TATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAA220.10339803543732669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)