FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845356
Total Bases146.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT14130.16714851494518287No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA10930.1292946403645328No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG10410.12314338574517718No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT9540.11285186359356295No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG8950.1058725554677556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)