FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136481
Total Bases23.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG4810.35243000857262186No Hit
CCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCAT4050.2967446018127065No Hit
GCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACT3060.2242070324807116No Hit
AGATGGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCAC2560.18757189645445155No Hit
ATGCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTA2280.1670562202797459No Hit
GGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGA2060.15093676042819146No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1980.14507513866398986No Hit
GTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACTGGAACTGCAC1970.14434243594346466No Hit
GACAAAAATGACCCCCATTTGTGTGACTTCATTGAGACACATTACCTGAA1820.13335189513558665No Hit
CATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGT1620.11869784072508262No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1570.1150343271224566No Hit
GTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCCGTGGGGTG1400.10257838087352818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)