FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19016
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2381.2515776188472865No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1520.7993268826251577No Hit
GGTATCAACGCAGAGTACATGGGGG1450.7625157761884729No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1360.715187210769878No Hit
GTATCAACGCAGAGTACATGGGGGG1260.6625999158603281No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1020.5363904080774086No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT880.4627681952040387No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT740.38914598233066894No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG710.373369793857804No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA670.352334875893984No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC590.31026503996634414No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC580.3050063104753891No Hit
GTATCAACGCAGAGTACATGGGGAG500.26293647454774927No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA480.2524190155658393No Hit
GTAAAGTGCTTCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGG470.2471602860748843No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT470.2471602860748843No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC470.2471602860748843No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG430.22612536811106435No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT430.22612536811106435No Hit
GGTATCAACGCAGAGTACATGGGGGG410.21560790912915437No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA400.2103491796381994No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT400.2103491796381994No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT400.2103491796381994No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT390.20509045014724445No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG390.20509045014724445No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA390.20509045014724445No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA380.19983172065628943No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT360.18931426167437948No Hit
GTATCAACGCAGAGTACATGGGAGG360.18931426167437948No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG340.1787968026924695No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC330.17353807320151451No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA320.16827934371055953No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT310.16302061421960454No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC310.16302061421960454No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG300.15776188472864955No Hit
TATCAACGCAGAGTACATGGGGAAG300.15776188472864955No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT290.15250315523769456No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC290.15250315523769456No Hit
ACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAG290.15250315523769456No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA290.15250315523769456No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT290.15250315523769456No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC280.1472444257467396No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA270.1419856962557846No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG270.1419856962557846No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC270.1419856962557846No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT270.1419856962557846No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG260.13672696676482962No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG260.13672696676482962No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG260.13672696676482962No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG260.13672696676482962No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG260.13672696676482962No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA260.13672696676482962No Hit
GTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAG260.13672696676482962No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC250.13146823727387463No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT250.13146823727387463No Hit
GAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCC240.12620950778291964No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC240.12620950778291964No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA240.12620950778291964No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA240.12620950778291964No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC230.12095077829196467No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA230.12095077829196467No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA230.12095077829196467No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT230.12095077829196467No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC230.12095077829196467No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA230.12095077829196467No Hit
CGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCT230.12095077829196467No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC220.11569204880100968No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT220.11569204880100968No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT210.11043331931005469No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG210.11043331931005469No Hit
GTATCAACGCAGAGTACATGGGGAAAG210.11043331931005469No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA210.11043331931005469No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG210.11043331931005469No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC210.11043331931005469No Hit
GTATCAACGCAGAGTACATGGAGAG200.1051745898190997No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT200.1051745898190997No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG200.1051745898190997No Hit
TGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTG200.1051745898190997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)