FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28654
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2440.8515390521393175No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1660.5793257485865848No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1580.5514064354016891No Hit
GTATCAACGCAGAGTACATGGGGGG1510.5269770363649054No Hit
GGTATCAACGCAGAGTACATGGGGG1270.4432190968102184No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1070.3734208138479793No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC880.3071124450338522No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG830.2896628742932924No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA740.2582536469602848No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC740.2582536469602848No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA700.24429399036783694No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC660.23033433377538912No Hit
GTATCAACGCAGAGTACATGGGGCG640.22335450547916522No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG630.21986459133105327No Hit
TATCAACGCAGAGTACATGGGGAATGCG620.21637467718294132No Hit
GTATCAACGCAGAGTACATGGGGTG610.21288476303482937No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT600.20939484888671736No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA580.2024150205904935No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT550.1919452781461576No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT530.18496544984993368No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA520.18147553570182173No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT430.15006630836881413No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA430.15006630836881413No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG420.14657639422070218No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT410.1430864800725902No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT400.13959656592447825No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC400.13959656592447825No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG390.1361066517763663No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT390.1361066517763663No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC390.1361066517763663No Hit
GTATCAACGCAGAGTACATGGGGAATGCG380.13261673762825435No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG380.13261673762825435No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC380.13261673762825435No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC380.13261673762825435No Hit
GGTATCAACGCAGAGTACATGGGGGG380.13261673762825435No Hit
GTATCAACGCAGAGTACATGGGGAG370.1291268234801424No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG370.1291268234801424No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT370.1291268234801424No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC360.12563690933203045No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT350.12214699518391847No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA350.12214699518391847No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG350.12214699518391847No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT350.12214699518391847No Hit
TACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACA340.11865708103580652No Hit
GTATCAACGCAGAGTACATGGGATG330.11516716688769456No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC320.11167725273958261No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC320.11167725273958261No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT310.10818733859147066No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA300.10469742444335868No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC300.10469742444335868No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT300.10469742444335868No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT290.10120751029524674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)