FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775607
Total Bases134 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT11170.14401623502624394No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA10870.14014829675338156No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG9240.1191324988041624No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT8530.10997837822505468No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG8280.10675509633100268No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA8060.10391860826423692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)