FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976496
Total Bases168.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT14460.14808048368861726No Hit
CCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCAT13030.13343628647736397No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG12800.1310809260867428No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG12260.12555094951745832No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA12020.12309318215333193No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG11140.11408136848486834No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT10600.10855139191558388No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT10430.10681047336599434No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG9990.10230456653176254No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGA9780.10015402008815193No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT9780.10015402008815193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)