FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008159872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008159872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8854
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGTCTTGACCCATGGCCGTGTCCGCCTGCTACTGAGTAAGGGGCATT430.4856562005873052No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC230.2597695956629772No Hit
GGGTTATGTGGTCCGAATCAGTGGTGGGAACGACAAACAAGGTTTCCCCA190.2145922746781116No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA180.20329794443189517No Hit
TGTTACAGACCAAGGAGAACTGGAGAAAGAAAGAGAAAATCAGTTCGTGG160.1807092839394624No Hit
GGCTTCACTGCTCAGGTGATTATCCTGAACCATCCAGGCCAAATAAGCGC150.169414953693246No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC140.1581206234470296No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA130.1468262932008132No Hit
GAACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACG130.1468262932008132No Hit
GCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCC130.1468262932008132No Hit
CCCTACTCTTGCTGGTAAAATCACTGGCATGTTGTTGGAGATTGATAATT120.1355319629545968No Hit
CTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGA120.1355319629545968No Hit
GGTCAGGAGTTCGAGACCAGCCTGACCAACATGGTGAAACCCCATCTCTA110.1242376327083804No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC110.1242376327083804No Hit
GGTGTCTGCAGAGTTAGCCCGTCTTTTCTGTTTAGCAGATGAAGAAACTG100.112943302462164No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACT100.112943302462164No Hit
GCCTGGCCAATATGGTGAAACCTGCCTCTACTAAAAATACAAAAAACTAG90.10164897221594758No Hit
ACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAG90.10164897221594758No Hit
GATGGTGGGCAGCATGGTGGGCGTCTACAACGGCAAGACCTTCAACCAGG90.10164897221594758No Hit
GCCCGATGCTCTCTGAAACATGTGCTGTGTCCACTCAGCGTTAGATGGAT90.10164897221594758No Hit
CACTTTGGGAGGCCGAGGCGGGTGGATTACAAGGTCAGGAGATCGAGACC90.10164897221594758No Hit
CTGTTGCCTTGGTAACCAGATTTGCAGGTGCAACTACCATTTTTGGCACT90.10164897221594758No Hit
CAGTGGCCTCTTTCGGCCCAGCCCAGTTCATGGCTCTCGGCGGCCTTCCC90.10164897221594758No Hit
CTTTTTGATGAAAAAGAAATTAAAAACATTTTCCTAGAAACAAATGTTAA90.10164897221594758No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC90.10164897221594758No Hit
CTATGGTGAAGTGACCAATGACTTTGTCATGCTGAAAGGCTGTGTGGTGG90.10164897221594758No Hit
CACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTA90.10164897221594758No Hit
GTGCTTATTAGGCCGTGCTCCAAAGTCATCGTCCGGTTTCTCACTGTGAT90.10164897221594758No Hit
CACCAAATGCAGAGAATTGTGAAAACCACTGATGAAACTGTCTTAAGAAA90.10164897221594758No Hit
GGAAAGGAGGTCAAAGAACACAAAGACTAGAGTGTCTGAAGGATCAAATC90.10164897221594758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)