FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18236
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2501.3709146742706733No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2061.1296336915990348No Hit
GGTATCAACGCAGAGTACATGGGGG1400.7677122175915772No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1210.6635227023470058No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA960.5264312349199386No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT940.5154639175257731No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT900.4935292827374424No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG870.47707830664619433No Hit
GTATCAACGCAGAGTACATGGGGGG760.4167580609782847No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT740.4057907435841193No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA720.394823426189954No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC720.394823426189954No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA690.37837245009870585No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC620.339986839219127No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC610.3345031805220443No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT600.3290195218249616No Hit
GTATCAACGCAGAGTACATGGGGAG580.31805220443079624No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA530.29063391094538277No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC530.29063391094538277No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT530.29063391094538277No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG510.27966659355121737No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG470.25773195876288657No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG470.25773195876288657No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA460.2522483000658039No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC450.2467646413687212No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT440.24128098267163853No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT430.23579732397455583No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC410.22483000658039043No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG390.21386268918622506No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT380.20837903048914236No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG380.20837903048914236No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC370.20289537179205966No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA360.197411713094977No Hit
GGTATCAACGCAGAGTACATGGGGGG360.197411713094977No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC350.1919280543978943No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT340.1864443957008116No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC320.17547707830664622No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA310.1699934196095635No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT310.1699934196095635No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC310.1699934196095635No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC300.1645097609124808No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG300.1645097609124808No Hit
TTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGT300.1645097609124808No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC290.15902610221539812No Hit
AGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATATTGAATATACT290.15902610221539812No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC290.15902610221539812No Hit
CATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTT290.15902610221539812No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA280.15354244351831542No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC270.14805878482123272No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA270.14805878482123272No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC270.14805878482123272No Hit
CTCCTAAGGCAACAGAAACTACTGGGGCGAGTATTCCCAATGTATGGTCA270.14805878482123272No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT260.14257512612415002No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA260.14257512612415002No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC260.14257512612415002No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA260.14257512612415002No Hit
CCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTAC260.14257512612415002No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT250.13709146742706735No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC250.13709146742706735No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA250.13709146742706735No Hit
GTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGA250.13709146742706735No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT250.13709146742706735No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT240.13160780872998465No Hit
TCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCT240.13160780872998465No Hit
CTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGG230.12612415003290195No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG230.12612415003290195No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG230.12612415003290195No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA230.12612415003290195No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG230.12612415003290195No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG230.12612415003290195No Hit
GTATCAACGCAGAGTACATGGGGTG220.12064049133581926No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT220.12064049133581926No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG220.12064049133581926No Hit
ATATAAAAGTGGAGGTTTTAGAAGAACAAGCTAAACTTACACTTTTAAAC220.12064049133581926No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGA220.12064049133581926No Hit
CGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGA220.12064049133581926No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT210.11515683263873656No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT210.11515683263873656No Hit
ACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTAC210.11515683263873656No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG210.11515683263873656No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG210.11515683263873656No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA210.11515683263873656No Hit
TAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAAT200.10967317394165388No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG200.10967317394165388No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC200.10967317394165388No Hit
GTATCAACGCAGAGTACATGGAGAG200.10967317394165388No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG200.10967317394165388No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG190.10418951524457118No Hit
GCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGGCGCATAG190.10418951524457118No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG190.10418951524457118No Hit
GAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAA190.10418951524457118No Hit
AATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGA190.10418951524457118No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC190.10418951524457118No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT190.10418951524457118No Hit
ATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTC190.10418951524457118No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG190.10418951524457118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)