FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21480
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4812.239292364990689No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4081.899441340782123No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2481.1545623836126628No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2120.9869646182495344No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1680.782122905027933No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1380.6424581005586593No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1140.5307262569832402No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1100.5121042830540037No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1090.5074487895716946No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT1020.4748603351955307No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC970.45158286778398504No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA920.42830540037243947No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG900.41899441340782123No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT820.3817504655493482No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT770.35847299813780265No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT750.34916201117318435No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT750.34916201117318435No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG710.33054003724394787No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG700.3258845437616387No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC690.32122905027932963No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA680.3165735567970205No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT670.3119180633147114No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC670.3119180633147114No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG670.3119180633147114No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG650.3026070763500931No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG650.3026070763500931No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC630.29329608938547486No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG630.29329608938547486No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA630.29329608938547486No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA630.29329608938547486No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA620.2886405959031657No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC610.2839851024208566No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA600.27932960893854747No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA590.2746741154562384No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG590.2746741154562384No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT590.2746741154562384No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT580.27001862197392923No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC570.2653631284916201No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA530.2467411545623836No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC520.24208566108007448No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG520.24208566108007448No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG510.23743016759776536No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA500.23277467411545624No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT500.23277467411545624No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG500.23277467411545624No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC480.22346368715083798No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG470.21880819366852886No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA470.21880819366852886No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC470.21880819366852886No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG460.21415270018621974No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT450.20949720670391062No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT450.20949720670391062No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG450.20949720670391062No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT440.2048417132216015No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT440.2048417132216015No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC440.2048417132216015No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC430.20018621973929238No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA420.19553072625698326No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT410.1908752327746741No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA410.1908752327746741No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC400.186219739292365No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC400.186219739292365No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG400.186219739292365No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA390.18156424581005587No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG390.18156424581005587No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG390.18156424581005587No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG380.17690875232774675No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT380.17690875232774675No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG380.17690875232774675No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG380.17690875232774675No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG380.17690875232774675No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT370.1722532588454376No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT370.1722532588454376No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC370.1722532588454376No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT360.16759776536312848No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT360.16759776536312848No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT350.16294227188081936No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT340.15828677839851024No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA340.15828677839851024No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC340.15828677839851024No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC340.15828677839851024No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC330.1536312849162011No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG330.1536312849162011No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA330.1536312849162011No Hit
TCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCG320.14897579143389197No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG320.14897579143389197No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA320.14897579143389197No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT320.14897579143389197No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA320.14897579143389197No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT310.14432029795158285No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT310.14432029795158285No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT310.14432029795158285No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA310.14432029795158285No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC310.14432029795158285No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC310.14432029795158285No Hit
AATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGA310.14432029795158285No Hit
GTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAA310.14432029795158285No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG310.14432029795158285No Hit
TGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAAC300.13966480446927373No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC300.13966480446927373No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC300.13966480446927373No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG290.13500931098696461No Hit
GCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAAT290.13500931098696461No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA290.13500931098696461No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT290.13500931098696461No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT280.1303538175046555No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT280.1303538175046555No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG280.1303538175046555No Hit
ACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTC280.1303538175046555No Hit
GTAAAGTGCTTCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGG280.1303538175046555No Hit
TCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGGGGCGAGTATT280.1303538175046555No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG280.1303538175046555No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC280.1303538175046555No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA280.1303538175046555No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT270.12569832402234637No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA270.12569832402234637No Hit
TCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGT270.12569832402234637No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC270.12569832402234637No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT260.12104283054003724No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG260.12104283054003724No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG260.12104283054003724No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC260.12104283054003724No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT260.12104283054003724No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC260.12104283054003724No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT260.12104283054003724No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT260.12104283054003724No Hit
GATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGT260.12104283054003724No Hit
CTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA250.11638733705772812No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA250.11638733705772812No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT250.11638733705772812No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG250.11638733705772812No Hit
TGTATGGTCAGAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTT250.11638733705772812No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT250.11638733705772812No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA250.11638733705772812No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC250.11638733705772812No Hit
GTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGG250.11638733705772812No Hit
GCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT240.11173184357541899No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC240.11173184357541899No Hit
ATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATT240.11173184357541899No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG240.11173184357541899No Hit
GTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTGGTAGACG240.11173184357541899No Hit
ATCTTTAATGTGGAGATAGGGTCTAAGGCAGATGTTGGTTCATCCGTCAA240.11173184357541899No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT230.10707635009310987No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA230.10707635009310987No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC230.10707635009310987No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC220.10242085661080075No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA220.10242085661080075No Hit
ATAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAAT220.10242085661080075No Hit
ATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAA220.10242085661080075No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA220.10242085661080075No Hit
TCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCT220.10242085661080075No Hit
GACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGG220.10242085661080075No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG220.10242085661080075No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG220.10242085661080075No Hit
CTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAA220.10242085661080075No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA220.10242085661080075No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT220.10242085661080075No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT220.10242085661080075No Hit
GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG220.10242085661080075No Hit
TAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACT220.10242085661080075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)