FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34005
Total Bases5.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGGG2050.6028525216879871No Hit
GGTATCAACGCAGAGTACATGGGGG1940.5705043375974121No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1380.4058226731363035No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1260.3705337450374945No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT870.2558447287163652No Hit
GTATCAACGCAGAGTACATGGGGAG790.23231877665049258No Hit
GGTATCAACGCAGAGTACATGGGGGG710.2087928245846199No Hit
GTATCAACGCAGAGTACATGGGGTG700.20585208057638582No Hit
GTATCAACGCAGAGTACATGGGGGGG670.19702984855168357No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA590.1735038964858109No Hit
TATCAACGCAGAGTACATGGGGGGG560.16468166446110866No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA530.15585943243640643No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC520.15291868842817233No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG510.14997794441993825No Hit
GTATCAACGCAGAGTACATGGGATG490.14409645640347007No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG470.1382149683870019No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA460.13527422437876782No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA460.13527422437876782No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT450.13233348037053375No Hit
GTATCAACGCAGAGTACATGGAGAG440.12939273636229964No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC430.12645199235406557No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA420.12351124834583148No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC390.11468901632112924No Hit
GTATCAACGCAGAGTACATGGGAGG380.11174827231289516No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC360.10586678429642699No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT360.10586678429642699No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA360.10586678429642699No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT350.10292604028819291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)