FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369501
Total Bases64.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT5020.13585890159972502No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4630.125304126375842No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG4310.11664379798701492No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT4120.11150172800614884No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG4050.10960728117109289No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT3980.10771283433603698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)