FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14110
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1120.7937632884479092No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1060.7512402551381999No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1010.7158043940467753No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA720.5102763997165132No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA550.38979447200566975No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA530.37562012756909996No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC480.3401842664776754No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG420.297661233167966No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT400.28348688873139616No Hit
CTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGA380.2693125442948264No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT350.24805102763997164No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA340.24096385542168677No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG340.24096385542168677No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA330.23387668320340185No Hit
GGTATTCCCGATCTGCCATGTATTCCAGAAAGGCCATGTACAAGAGGAAG320.22678951098511693No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA310.21970233876683204No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG310.21970233876683204No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA280.1984408221119773No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA260.1842664776754075No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG250.1771793054571226No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA230.16300496102055279No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT230.16300496102055279No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG220.1559177888022679No Hit
GTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAG220.1559177888022679No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC210.148830616583983No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA210.148830616583983No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA210.148830616583983No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT200.14174344436569808No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA200.14174344436569808No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA200.14174344436569808No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC190.1346562721474132No Hit
GAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATATTGAATATAC180.1275690999291283No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC180.1275690999291283No Hit
CTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAATAAG180.1275690999291283No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT180.1275690999291283No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT170.12048192771084339No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA170.12048192771084339No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA170.12048192771084339No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT160.11339475549255847No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG160.11339475549255847No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT160.11339475549255847No Hit
CAGCTGTACAGTGCGCGCCAGCGGCGGCGGCTGAACCGGGGCCTGCGGCG160.11339475549255847No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGA160.11339475549255847No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA160.11339475549255847No Hit
GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA160.11339475549255847No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG150.10630758327427356No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC150.10630758327427356No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA150.10630758327427356No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG150.10630758327427356No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC150.10630758327427356No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC150.10630758327427356No Hit
CACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAATA150.10630758327427356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)