FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236
Total Bases202.6 kbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTGAAGGGGATCAAGGGACGGCTGAACAGACTTCCCGCTGCTGGTGT80.6472491909385114No Hit
TTTCCGCACCTGGCCGAAAAATTATTTTAAAAGGATCAAAACTATGCAGT70.5663430420711975No Hit
GTATAGTACCTAGTACCCAATAAGTGCTCAATAAATGTGTTATGCTGGCC70.5663430420711975No Hit
GTCAAAAGCTTAGATACACCACAAGGCAGCAGCTAAGCTTAAAGAAGTGC60.48543689320388345No Hit
GATCACCTGAGGTCAGGAGTTCGAGACCAGCTTGACCAACATGGAGAAGC60.48543689320388345No Hit
ATGTATAGATGAAAAAAGGGGTCCCTGTGTCTAAATTTTGTCCCTTCTTG50.40453074433656955No Hit
GCTAAGTAGCTCTATTATAATACTTATCCAGTGACTAAAACCAACTTAAA50.40453074433656955No Hit
GCAGATACCTTTACACCCTGGTCATCACTGACAAAGAGAAGGCAGAGAAA50.40453074433656955No Hit
ACCCTATACTTGCAGTGTTTGTGGGAAAGGCTTCTCAAGGCCTGACCACT50.40453074433656955No Hit
ACTATGCCTAGAAGGAATAATACTATCGCTGTTCATTATAGCTACTCTCA50.40453074433656955No Hit
TCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCC50.40453074433656955No Hit
CATATGATGTGGTGGTTCTACCAGGAGGTAATCTGGGCGCACAGAATTTA40.3236245954692557No Hit
CCCTATTACTATCCTTCCTCATCCTAGCAATAATCCCCATCCTCCATATA40.3236245954692557No Hit
TGTCTACACTGGATAATCATTCCCTGGCCATATCAGTCATCATGATGGTG40.3236245954692557No Hit
TCCGAACGCTGCAGGCCGGCCAGGTCCCTGGGCGTCCAGGCCTGGCCTAC40.3236245954692557No Hit
CAATAACATCCGCTCCTAAGGCAACAGAAACTACTGGGGCGAGTATTCCC40.3236245954692557No Hit
GAATCTGGGCCTAACAATTTTATCTGAAGACAGTTCTAAATCAAGGGTTT40.3236245954692557No Hit
GATGTGAGCACTGTTGCTCAGGAGTGACAAGTTGCTAATATGCAGAAGGG40.3236245954692557No Hit
CTTACATAGTTTTTGGGGAAGCCAAGATCGAAGATTTATCCCAGCAAGCA40.3236245954692557No Hit
TATCCACTCGCCACAGTTATCAGCTGCCATTGCTCCCTGTCTGTTTCCCC40.3236245954692557No Hit
ACCAAACACAATGCAGAGCTGGCAAGCTATGAGAGCCAGATTGCCAAGCT40.3236245954692557No Hit
GAATTGGCCTCATGATGCATCAGGAGTTGTCTAAGGGAAATCAGGTGACT40.3236245954692557No Hit
GTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATCCTTCCTCAT40.3236245954692557No Hit
TATTTATCCTCTCCATGATGTCTTCGTTAGAAAAGTAAAAATGCTGAAGA40.3236245954692557No Hit
TCACTGGACGCCACAGGGGCAAGAGGGTGGTTTTCCTGAAGCAGCTGGCT40.3236245954692557No Hit
GGCTGAGGCAAGCAGATTGCCTGAGGTCAGGAGTTCGAGACCAGCCTGGC40.3236245954692557No Hit
CACAATAATAGTGGGAGATTTTAATACCACACTGTCAGTATTAGACAGAT40.3236245954692557No Hit
CTGATATAGACACAAGCTGCAAAGATGTCCTGTCTTGCACAGGCACTTCC40.3236245954692557No Hit
ATAAAAGTTCATCCAGAGGCCAGGCGGTGGCTCACGTCTGTAATCCCAGC30.24271844660194172No Hit
AGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGAAGGCCAAGGTG30.24271844660194172No Hit
GGCTGGTCTCGAACTCCTGGCCTCACGTGATCTGTCCACTTCAGCCGTCC30.24271844660194172No Hit
TCCTCCAACCCTCTGAGATGCTGACACCACTATCATTAAATAACTTTTGT30.24271844660194172No Hit
CACTAGTGGTTACTTTCTGGGAAGGACTAAGGTGGCAGAGGTTGGGAGGG30.24271844660194172No Hit
TTCAAGAAGCGTGCACCTCGGGCACTCAAAGAGATTCGGAAATTTGCCAT30.24271844660194172No Hit
GGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACGAGGTGGCCGA30.24271844660194172No Hit
ATATGATAGTCTGCGCAGCGTATGCACACGAACTGCCAAAATATGGTGTG30.24271844660194172No Hit
TTGCAAACACAGGTCAGTCTGTGCAAAGGCCCTGAGACACAAGGAGCCTA30.24271844660194172No Hit
CACACACACACATATATATATATACATATCACAGTTTAGGTAGAGAGTAC30.24271844660194172No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA30.24271844660194172No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC30.24271844660194172No Hit
GAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAGACAAT30.24271844660194172No Hit
GTTCAAGTTTCCTGGCCGCCAGAAGATCCACATCTCAAAGAAGTGGGGCT30.24271844660194172No Hit
TCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA30.24271844660194172No Hit
GTGCTGGGTGCAGATGCAGTGTGGCTCTGGATAGCACCTTATGGACAGTT30.24271844660194172No Hit
TCCACATCTCAAAGAAGTGGGGCTTCACCAAGTTCAATGCTGATGAATTT30.24271844660194172No Hit
GTCTGGGCCAAAGGAATAAGGAATGTGCCATACCGAATCCGTGTGCGGCT30.24271844660194172No Hit
GGCTTCTAATGACCCAGATATGGAAACAGAAGACAAAATTGTAAGCCAGA30.24271844660194172No Hit
GATATGCTCATGTGGTGTTGAGGAAAGCAGACATTGACCTCACCAAGAGG30.24271844660194172No Hit
GTACATTTGTTGCAGTTGATGAACCTACATTGACATATTATCACCCAAAG30.24271844660194172No Hit
TCCTTGTTTCCTCCTGTGCTGAGCCCTTTCCTTGTGTCCATTCCACCAGA30.24271844660194172No Hit
TCGTCACAGTGGGCGAGTGCCGGCCTCTGAGCAAGACAGTGCGCTTCAAC30.24271844660194172No Hit
GGTCATTGCTGGCCCTCAGGATTCCCCCTTTGAGGGAGGGACTTTTAAAC30.24271844660194172No Hit
CCTAGGAGCTGCCATGGGAGTTACCAAAAATATGTGAATAAACTGAATGC30.24271844660194172No Hit
ACAGCACCACACAGCTCACGACAACACCACACAGCTCACGACAGCACCAC30.24271844660194172No Hit
GTCCAAGGCCATTTTTGCTGGCTATAAGCGGGGTCTCCGGAACCAAAGGG30.24271844660194172No Hit
AAACTAGTATGACCGGAGGCTCAATTTAGAAGATGCAGCATCTGAAAACC30.24271844660194172No Hit
GGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA30.24271844660194172No Hit
GTGTAAGGGTCCAGCTGATCAAGAATGGCAAGAAAATCACAGCCTTTGTA30.24271844660194172No Hit
ATTCTAAACCTATTCAAAATATGCTTACTCACAGAGAGGTCTCACAAGTA30.24271844660194172No Hit
GTAGGCTGTACCCTCAAAGTGCTAAGGAGAGCTTCTGTCCTCGAAAATCT30.24271844660194172No Hit
CCTTAACCCCCCATGCAGTGCTGAGAGGTCAAACCTTTGAGAGGTGACTA30.24271844660194172No Hit
GTGTTGTACTCGTAAAACAAGGATCATCGATGTTGTCTACAATGCATCTA30.24271844660194172No Hit
CGCCCAGGGAAAGCGGCGTTATGACAGGAAGCAGAGTGGCTATGGTGGGC30.24271844660194172No Hit
CCAGAACACACTGTCAGTTCCATGGACCTAGGATTATAAAATCCTGTAAG30.24271844660194172No Hit
ACGCAGAGTACATGGGGCTCTCTTCCTCGGCGCTGCCTACGGAGGTGGCA30.24271844660194172No Hit
CCTGGAGCCAGATGGCAAGAGGGTGACAGCTTCCTTTCCTGTGTGTACTC30.24271844660194172No Hit
ATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTA30.24271844660194172No Hit
GAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGGAGCGCCCGAATC30.24271844660194172No Hit
CAAGAAGATTTATAAGATTGGCCAGGGCTACCTTATCAAGGACGGCAAGC30.24271844660194172No Hit
ACATTGGAGATTGGTTGCTTTCTAAAACTGAAGGAGAAGCCCATGAAGAG30.24271844660194172No Hit
CTGTGGTCCCAGCTACTGGGGAAGCTGAGGCAGGAGAATGGCGTGAACCC30.24271844660194172No Hit
GTCCAGGTGCCACAGGCAGCCCTGGGACATAGGAAGCTGGGAGCAAGGAA30.24271844660194172No Hit
GGTTTATGCCTGTAATCCCAGCACTGTGGGAGGCTGAGGTGGGTGGATCA30.24271844660194172No Hit
GACAAGTTGCTAATATGCAGAAGGGATGGGTGATACTTCTTGCTTCTCAT30.24271844660194172No Hit
CCTATGTACCTGTTACCACTTTCAAAAATCTACAGACAGTCAATGTGGAT30.24271844660194172No Hit
CATGATGGAGGAGATGTATAAGAAAGCTCATGCTGCTATACGAGAGAATC30.24271844660194172No Hit
TCCCAAAGTGCTGGGCTTACAGGCCTGAGCCACAGTGTCTGGCCCACAAA20.16181229773462785No Hit
GTGTATAACATTCACCTGGCTGTCAACTTCTTGGTGTCATTGCTCAAGAA20.16181229773462785No Hit
GAGCTGCAACAAGGTGGAAACAAGGGCTGGAGCTGCGTTTGTTTTGCCAT20.16181229773462785No Hit
ACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC20.16181229773462785No Hit
CACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGG20.16181229773462785No Hit
AATTATGATTAAATGATAATGGGCCTGTTATTATTAGAGTGTTTTTCGCC20.16181229773462785No Hit
ATATATCTACCTCCTTTTGCTTTTTTATTTGCTAAACTAGCTCATACTGT20.16181229773462785No Hit
TGAGCACACAGAGCAGCATCTGATGCCCTGTGGTGTTTGGCATGTGCCCC20.16181229773462785No Hit
GACTTCCAAGAGTACTGTGTCTTCCTGTCCTGCATCGCCATGATGTGTAA20.16181229773462785No Hit
GCATCCAACTGTGATGAGCCTATGTATGTCAAGTTGGTGGAGGCCCTTTG20.16181229773462785No Hit
TCTGTATCCAGCTCCTGAACAAGACATGGAAGGAAATGAGGGCAACTTCT20.16181229773462785No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT20.16181229773462785No Hit
GCTATGGTGGGCAAACTAAGCCGATTTTCCGGAAAAAGGCTAAAACTACA20.16181229773462785No Hit
CTTCAACGCCCCAAGTTATTTCATCAGGCTAAGAACTTCTCCGAGAAACG20.16181229773462785No Hit
GTGTATGGAGGAGGGGCTGCTGAGATATCCTGTGCCCTGGCAGTTAGCCA20.16181229773462785No Hit
AGGCTGCAGTGAGCCATGATTGTACCACTGCACTCCAGCAGCCTGGTCAA20.16181229773462785No Hit
GACCAGTAGACGCTCCTCTACTCTTTGAGACATCACTGGCCTATAATAAA20.16181229773462785No Hit
GGAAGGTAGAAATTAAGAAGAGATTCTTTACCTGAAATCTTGCCAGAGAT20.16181229773462785No Hit
GGATGGACTGGCAGAAGAAGAGGCTGAAAAAACCTCTAGGAGTCATCATA20.16181229773462785No Hit
CAAAGTAAAGATAACAAAAAAGTCACAAAAGCCAAGGGATAAAAAGCAAT20.16181229773462785No Hit
GAATATTTATGTGATATTTATAGTCTGTGAAGAAAACCCTGGGGAACTGC20.16181229773462785No Hit
GCCATCTCCTTCTCGGCATCATGGCCGCCCTCAGACCCCTTGTGAAGCCC20.16181229773462785No Hit
AGCTCAGGGAGGTCAAGGCTGCAGTGAGCCATGACGATACCGCTGCACTC20.16181229773462785No Hit
GTCTAAGTTTCCCCTTTTAAGGTTTCAACAAATTTCATTGCACTTTCCTT20.16181229773462785No Hit
GGCATGGCCAGGTGCCCCCTGGGTTGAAACCAAAGAGAGGCCATCACCTG20.16181229773462785No Hit
CTGTTGTACCGTGGTGACGTGGTTCCCAAAGATGTCAATGCTGCCATTGC20.16181229773462785No Hit
GATTAATACAGATCTTAGCAGAATCTTGAAAAGCCCAGAGATCCAAAGAG20.16181229773462785No Hit
ATGCTATACAGGTGCAACTCAATCCCCGTTAATAAAAACCAATGTAGGTA20.16181229773462785No Hit
GCCTGGCCAACATGGCGAAACCCTACCTCTACTAAAAATACAAAAATTAG20.16181229773462785No Hit
ATCCTTATCAGATCATTGTTGTATTCTAAGGAGTGACGTATTTTTCACCT20.16181229773462785No Hit
GTACATGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGT20.16181229773462785No Hit
GGGCTCTGGAGAAGATTGACCTTAAGTTCATTGACACCACCTCCAAGTTT20.16181229773462785No Hit
GAAGGATGTAAAGGATGGAAAATACAGCCAGGTCCTAGCCAATGGTCTGG20.16181229773462785No Hit
AAATCAAGAAACTTGGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGC20.16181229773462785No Hit
GGTTAAGTCTGCTAAATTACGGTATGCATTATCTGGTGACTATTTGTGCC20.16181229773462785No Hit
GGGTAGGCCTTTGTAAAATTGACAGAGAGGGGAAACCCCGTAAAGTGGTT20.16181229773462785No Hit
TCCACATGTTGCTAGAGGGGCTTAGAGAACTACAAGGCCTGCAGAATTTC20.16181229773462785No Hit
GTAAGGGACCTTCGATTAAAAAAAAAAAAAGACAAT20.16181229773462785No Hit
GGTGGGATGAAGGTAAAGGCAGACCGAGATGAATCCTCACCATATGCTGC20.16181229773462785No Hit
AAGTTATTTCATCAGGCTAAGAACTTCTCCGAGAAACGCACAAGAAGGCA20.16181229773462785No Hit
CTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCT20.16181229773462785No Hit
ACCCTGTTTCAAAAAAAACAAAAATTCATTATAAATCACAGAACCTGACA20.16181229773462785No Hit
GAGTTCAAGGCTGCTGTGAGCCAAGATCATACCACTGCACTCCACCCTGG20.16181229773462785No Hit
GATTGAGGCCCTGCGCAGGGCCAAGTTCAAGTTTCCTGGCCGCCAGAAGA20.16181229773462785No Hit
GTCGTATACCTGAGGTGCACCGGAGGTGAAGTCGGTGCCACTTCTGCCCT20.16181229773462785No Hit
GACAAGAATAGAGAAAAAAAATGAAACAAGCCTCTAAGAAATATGAGTCT20.16181229773462785No Hit
TCCCTAAAACCCGCCGGACTTTCTGTAAGAAGTGTGGCAAGCACCAACCC20.16181229773462785No Hit
TCTTGAAGCAAGGTTCCAAGCACAAAACTAGTATGACCGGAGGCTCAATT20.16181229773462785No Hit
GGCTGGTCTTGAACTCCTGGCCTCAAGTGATCCGCCCACCTCGGTCTCCC20.16181229773462785No Hit
ATTTAGAAGATGCAGCATCTGAAAACCTTTACCCCAGGAAAGGAGGGGTG20.16181229773462785No Hit
GGCTTCACTGCTCAGGTGATTATCCTGAACCATCCAGGCCAAATAAGCGC20.16181229773462785No Hit
CCTAATACTATGAAACCATTAAAAATATTACAGAAGACCCAGCCTGGCAA20.16181229773462785No Hit
ATATGGCACTCTGCCTAACACACGTTAATAACTCAATACATGTTAGCAAT20.16181229773462785No Hit
TGTAAAATTCCACATGTATTAAAATGTACAAAATGTTTTGTTAATAAAAT20.16181229773462785No Hit
GAGAGGTAGCAACTAGGTAAACTCCTTATAAAAAGCAAATACCTGGATTT20.16181229773462785No Hit
AAATGGACGTCTTCTTTGCAAGTAGGAGGGACATTTGCAGAAAACTGCAG20.16181229773462785No Hit
GAGCGGGACTCTGTCTCAAAAACAAAAGTTGAAAATAGGGCCAAGTGCGG20.16181229773462785No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC20.16181229773462785No Hit
GGCCAAGCAATGGGGCTGGACACAAGGTCGGTGGCCCAAAAAGAGTGCTG20.16181229773462785No Hit
GGGAAGAGAAAAAGGATGGACTCTGTTACCCACAGCCTCTAATACCTTCA20.16181229773462785No Hit
AAATAACACAAATAATAATATCCTTCGTTATAACCACAATGAGATAAGTA20.16181229773462785No Hit
GTATGGGTGTAACACAATTTATTTAATCACTTCCTTATGGCCCTTCCCAG20.16181229773462785No Hit
CTCACAATATGGGGAGATTGTTAACATTAATCTCGTGCGGGACAAGAAAA20.16181229773462785No Hit
CGTCCGCCGAGGGGCGCCAGCCTGAAGCTGCCAGGCCCTGAGGTTCAGAC20.16181229773462785No Hit
GAGGGGAGGAGAGGGCAAGGGAGGGGTG20.16181229773462785No Hit
CTTTGTGGGAGTCTGGGTCGGCACACTGTCAGTACATCAGGCACATGGGC20.16181229773462785No Hit
GGCGGGAGAATGGCGTGAGCCCGGGAGGCGGAGCTTGCAGTGAGTCGAGA20.16181229773462785No Hit
GCCATCCTACGCCGTAGCCGTCCAGAGACTGGCAGGCCTCGGCCTAAAGG20.16181229773462785No Hit
GATGTATCCATATTATAGATTAATAACACTCATTAAAAATAAAGGTTGCC20.16181229773462785No Hit
CTCCTTCGGCCTTCTTCCTCTTCTGCTCTGAGTATCGCCCAAAAATCAAA20.16181229773462785No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT20.16181229773462785No Hit
CCTTCGGTGCTGCCCTGGGCCGCCGAGCCTTCATCCCTCTCCCCAGCGCT20.16181229773462785No Hit
GAAAAGTAGGAAGCAGAAGAAAAAAGACAAGCTAGGAAACAAAAAGCTAA20.16181229773462785No Hit
GCTCACGCCTATAGTCCCAGCCCTTTGGAAGGCTGAGGTGGGAGGACTGC20.16181229773462785No Hit
GCTGCGGCTTGAGTCCAGGAGTTGGAGGCTGCAGTGACCTGTGATTGCAC20.16181229773462785No Hit
CTGTCTGAAGTTCGAGACTTAGACTTTGCCAATGATGCCAATAAAGTACT20.16181229773462785No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC20.16181229773462785No Hit
CTCTAATATATCCCCAGTCTAAGGCATTTAAAATTAAACAGCTCTTCAAC20.16181229773462785No Hit
GCAAAAAAGGGAGTTGTCAAGGCTGAAAAAAGCAAGAAAAAGAAGGAAGA20.16181229773462785No Hit
GTACATGGAACCTTATGGGACACAAACAGGAGAGTCAGGCAATGTGAAAA20.16181229773462785No Hit
TTCCAGCTCCGTTGGCGTTGTGGAGAGAGCGGCACGTTTGGCCAAGCTTC20.16181229773462785No Hit
AATCTCCATCTCAAAAAAAAAAAAAAAGTTACAAGCCAAGACATCCCACC20.16181229773462785No Hit
GTCCTATGGGGAGCAGTTTGGGGGCGGCCGGCAGCAGGAGCCTGGGAAAG20.16181229773462785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)