FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14624
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG6074.150711159737417No Hit
GTATCAACGCAGAGTACATGGGGGG3242.2155361050328226No Hit
GTATCAACGCAGAGTACATGGGGAG1541.0530634573304158No Hit
GGTATCAACGCAGAGTACATGGGGGG1260.861597374179431No Hit
GTATCAACGCAGAGTACATGGGGAAG850.5812363238512035No Hit
TATCAACGCAGAGTACATGGGGAAG800.5470459518599562No Hit
GTATCAACGCAGAGTACATGGAGAG750.512855579868709No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC710.4855032822757112No Hit
GGTATCAACGCAGAGTACATGGGGAG630.4307986870897155No Hit
GTATCAACGCAGAGTACATGGGGGGG480.3282275711159737No Hit
GTATCAACGCAGAGTACATGGGGAAAG460.31455142231947486No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC460.31455142231947486No Hit
TATCAACGCAGAGTACATGGGGGGG440.30087527352297594No Hit
GTATCAACGCAGAGTACATGGGGTG430.2940371991247265No Hit
TATCAACGCAGAGTACATGGGGAAAG380.2598468271334792No Hit
GTATCAACGCAGAGTACATGGGAGG380.2598468271334792No Hit
GGTATCAACGCAGAGTACATGGGAG370.25300875273522977No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC320.2188183807439825No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC300.20514223194748357No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC270.18462800875273525No Hit
TATCAACGCAGAGTACATGGGGAGAG270.18462800875273525No Hit
GTATCAACGCAGAGTACATGGGGGAG260.17778993435448578No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC260.17778993435448578No Hit
TATCAACGCAGAGTACATGGGGGAG260.17778993435448578No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA260.17778993435448578No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA260.17778993435448578No Hit
TATCAACGCAGAGTACATGGGAGGG250.17095185995623632No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT230.15727571115973743No Hit
GGTATCAACGCAGAGTACATGGGGGGG220.15043763676148797No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA220.15043763676148797No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC200.13676148796498905No Hit
GTATCAACGCAGAGTACATGGGAGGG200.13676148796498905No Hit
TATCAACGCAGAGTACATGGGAGAG180.12308533916849015No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG180.12308533916849015No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA180.12308533916849015No Hit
TATCAACGCAGAGTACATGGGGGGAAAAAAAGG180.12308533916849015No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG170.11624726477024071No Hit
GTATCAACGCAGAGTACATGGGATAGAAGCCGATACCGTGAAAGAAGAAA170.11624726477024071No Hit
CGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGAT170.11624726477024071No Hit
GGTATCAACGCAGAGTACATGGAGAG170.11624726477024071No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC160.10940919037199125No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG160.10940919037199125No Hit
GTATCAACGCAGAGTACATGGGGGAAAAAAG160.10940919037199125No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA160.10940919037199125No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA160.10940919037199125No Hit
GTATCAACGCAGAGTACATGGGAAG160.10940919037199125No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA160.10940919037199125No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA150.10257111597374179No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT150.10257111597374179No Hit
GTATCAACGCAGAGTACATGGGGGGAAAAAAAGG150.10257111597374179No Hit
GTATCAACGCAGAGTACATGGGAAGG150.10257111597374179No Hit
TATCAACGCAGAGTACATGGGGAAAAAG150.10257111597374179No Hit
GGTATCAACGCAGAGTACATGGGGAAG150.10257111597374179No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG150.10257111597374179No Hit
GTATCAACGCAGAGTACATGGGGAGAG150.10257111597374179No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)