FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21574
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG1900.8806897191063318No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1780.8250672105311949No Hit
GTATCAACGCAGAGTACATGGGGGGG1630.755539074812274No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1590.7369982386205618No Hit
TATCAACGCAGAGTACATGGGGGGG1460.6767405209974969No Hit
GTATCAACGCAGAGTACATGGGGGG1310.6072123852785761No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1270.5886715490868638No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA730.33837026049874847No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT670.3105590062111801No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT610.2827477519236118No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG590.27347733382775563No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT580.26884212477982755No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC570.26420691573189947No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA560.25957170668397145No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC510.23639566144433113No Hit
GGTATCAACGCAGAGTACATGGGGGG490.22712524334847503No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC480.22249003430054698No Hit
GGTATCAACGCAGAGTACATGGGGGGG480.22249003430054698No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC480.22249003430054698No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT460.21321961620469082No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA450.20858440715676277No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA420.1946787800129786No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT410.19004357096505053No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC410.19004357096505053No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG400.18540836191712246No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG390.1807731528691944No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG360.16686752572541022No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC360.16686752572541022No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT350.16223231667748217No Hit
GTATCAACGCAGAGTACATGGGATG350.16223231667748217No Hit
GTATCAACGCAGAGTACATGGGGTG340.1575971076295541No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC330.15296189858162604No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG320.14832668953369796No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA310.1436914804857699No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG310.1436914804857699No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG310.1436914804857699No Hit
GTATCAACGCAGAGTACATGGGGAG300.13905627143784186No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT290.13442106238991378No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG290.13442106238991378No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG280.12978585334198572No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT270.12515064429405767No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA270.12515064429405767No Hit
ATAATATCCTTATCTTGAGACGCATAGCAAATGTAGGAGAGAGAGGTTAA270.12515064429405767No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT250.11588022619820153No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA250.11588022619820153No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA250.11588022619820153No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG250.11588022619820153No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG250.11588022619820153No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT240.11124501715027349No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC240.11124501715027349No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT240.11124501715027349No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC240.11124501715027349No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA240.11124501715027349No Hit
GTATCAACGCAGAGTACATGGGGGGGACCTCC240.11124501715027349No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC240.11124501715027349No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA240.11124501715027349No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC240.11124501715027349No Hit
AATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGA230.10660980810234541No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC230.10660980810234541No Hit
GGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGG220.10197459905441734No Hit
GTATCAACGCAGAGTACATGGGATGG220.10197459905441734No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA220.10197459905441734No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC220.10197459905441734No Hit
CTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTAC220.10197459905441734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)